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Expression Profiling and Function Analysis Identified New Cumulus Cells-Expressed Genes and miRNAs Predictive of
Min Zhang1, Meng-Meng Wang1, Fa-Li Zhang1
1College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an City 271018, China.
Cells
|June 11, 2025
Summary
Researchers identified specific genes and miRNAs in cumulus cells (CCs) that directly correlate with oocyte developmental potential (DP). These findings offer new biomarkers for selecting more competent oocytes and embryos, improving IVF outcomes.
Area of Science:
- Reproductive Biology
- Molecular Biology
- Genetics
Background:
- Cumulus cells (CCs) express genes and miRNAs influencing oocyte and embryo quality.
- Existing markers for oocyte developmental potential (DP) are limited.
- A need exists to identify CC-expressed molecules directly correlated with DP.
Purpose of the Study:
- To identify and validate CC-expressed genes and miRNAs linked to oocyte developmental potential (DP).
- To establish reliable biomarkers for selecting competent oocytes and embryos.
Main Methods:
- Established mouse CC models from cumulus-oocyte complexes (COCs) with varying DP.
- Performed mRNA/miRNA sequencing and functional analyses.
- Utilized a well-in-well culture system for tracking and validation.
Main Results:
- Identified DP-beneficial genes: Spp1, Fn1, Sdc1, Ngf.
- Identified DP-detrimental genes: Fos, Jun.
- Identified DP-detrimental miRNAs: miR-7686-5p, miR-133a-3p, novel-miR-239, novel-miR-193, miR-339-5p.
- Validated Spp1, Ngf, Jun, miR-7686-5p, novel-miR-239, and miR-339-5p in relation to DP.
Conclusions:
- New sets of CC-expressed genes and miRNAs directly correlate with oocyte DP.
- These molecules show promise as biomarkers for selecting competent oocytes and embryos.
- Findings may enhance assisted reproductive technologies.
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