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Updated: Jun 21, 2026

Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes
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Hepatocellular Metabolic Profile: Understanding Post-Thawing Metabolic Shift in Primary Hepatocytes In Vitro

Salvator Palmisano1, Joshua D Breidenbach1, Brett R Blackwell1

  • 1Biochemistry and Biotechnology Group (B-TEK), Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.

Cells
|June 11, 2025
PubMed
Summary

Primary human hepatocytes (PHHs) show significant metabolic changes within five hours post-thawing. Early responses are conserved, but later changes reveal acetaminophen-specific effects, impacting liver model design.

Keywords:
LC-MS/MS metabolomicsin vitro liver modelsliver metabolismmetabolic profilingpost-thawing hepatocyte stabilityprimary human hepatocytes (PHHs)

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