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Modified Methylation Following Electrostimulation in a Standardized Setting-Complementing a Transcriptomic Analysis.

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Epigenetics

Background:

  • Electrical stimulation (ES) is utilized in clinical therapies and research for tissue regeneration, wound healing, and inflammation control.
  • ES influences DNA demethylation, a key process in nerve regeneration and cellular repair.
  • The broader impact of ES on cellular functions, especially in inflammation and wound healing, requires further investigation.

Purpose of the Study:

  • To investigate the differential effects of ES on DNA methylation.
  • To explore the correlation between ES, DNA methylation, and age acceleration using a mitotic clock.
  • To understand the mechanistic details of ES in controlling senescent processes.

Main Methods:

  • Utilized a 3D in vitro model with human fibroblasts and keratinocytes in a collagen matrix.
  • Applied direct current (DC) stimulation at 1 V (DC1).
  • Analyzed transcriptomic and metabolomic profiles.
  • Investigated DNA methylation patterns and correlated them with a mitotic clock for age acceleration.

Main Results:

  • ES differentially affects DNA methylation in inflamed versus non-inflamed samples.
  • Direct current stimuli at 1 V (DC1) show potential involvement in controlling senescence associated with mitosis and inflammation.
  • Modulation of transcriptomic and metabolomic profiles by ES was observed in the in vitro model.

Conclusions:

  • Electrical stimulation has a differential impact on DNA methylation in inflamed and non-inflamed cellular models.
  • DC1 may play a role in regulating senescence, mitosis, and inflammation, warranting further mechanistic studies.
  • ES impacts cellular functions beyond direct tissue repair, influencing epigenetic regulation and aging processes.