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Updated: Jun 12, 2025
11:02
Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
Published on: November 29, 2024
483
Metabolite Analog-Induced Proteome Changes.
Molly Kennedy1, Nikhil Gandikota1,2, Victoria Pereira1
1Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
Methods in Molecular Biology (Clifton, N.J.)
|June 11, 2025
Summary
Metabolite analogs, structurally similar to natural metabolites, are key tools for studying metabolic pathways and developing treatments for diseases like cancer. This work details their preparation, handling, and proteomic analysis for research applications.
Area of Science:
- Biochemistry
- Molecular Biology
- Pharmacology
Background:
- Metabolites are small molecules essential for cellular functions.
- Metabolite analogs are compounds that mimic or inhibit natural metabolites.
- These analogs are crucial for studying metabolic pathways and developing therapeutics.
Purpose of the Study:
- To detail the preparation and handling of metabolite analogs.
- To outline a protocol for protein extraction after analog treatment.
- To describe the proteomic analysis of cellular responses using Proteome Discoverer software.
Main Methods:
- Preparation and storage of metabolite analogs.
- Protein extraction from treated samples.
- Proteomic analysis using Proteome Discoverer software.
Main Results:
- The study provides a detailed protocol for metabolite analog handling and sample preparation.
- It outlines a method for analyzing proteome alterations induced by metabolite analogs.
- The described methods facilitate the investigation of cellular pathway modulation.
Conclusions:
- Metabolite analogs are valuable tools for elucidating metabolic mechanisms.
- This protocol enables the study of how analogs affect cellular pathways like PTEN and mTOR.
- The methodology supports research into potential treatments for cancer and metabolic disorders.
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