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Published on: February 14, 2012
Polyamine Metabolism in Optic Nerve: Spatial Metabolomics with AP/MALDI Mass Spectrometry Imaging
Sean Meehan1, Caleigh O'Connor2, Eugene Moskovets2
1Bascom Palmer Eye Institute, Miller School of Medicine, University of Miami, Miami, FL, USA.
Abstract:
Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is widely recognized as a leading method for studying spatial omics. Spatial multiomics platforms are most effective when they integrate multimodal datasets to provide a comprehensive understanding of biological contexts. By combining approaches like metabolomics and lipidomics, the throughput of spatial analysis methods can be significantly enhanced. In this particular protocol, polyamine pathway-related metabolites and lipids from a mouse optic nerve are imaged using atmospheric pressure MALDI.

