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Updated: Jun 12, 2025

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
Published on: March 14, 2013
Direct Colony Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Assay for Targeted
Nivedita Bhattacharya1,2,3, Deepanwita Banerjee4, Caleigh O'Connor5
1MassTech Inc., Columbia, MD, USA. nivedita@apmaldi.com.
Abstract:
Atmospheric pressure matrix-assisted laser desorption/ionization (AP/MALDI) mass spectrometry (MS) allows for highly sensitive and specific detection and relative quantification of metabolites of interest, with the key benefit of minimal sample preparation requirements for complex biological samples such as bacteria. Violacein is a violet-colored pigment produced by the bacterium Chromobacterium violaceum. In this chapter, we detail a high-throughput, high-resolution (HR) MS-based, targeted metabolite profiling protocol for analyzing violacein and its intermediates directly from bacterial colonies. This technique applies to a wide range of metabolites regulated by operon control and is suitable for a molecular phenotyping platform.
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