Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

282
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI spectrometry is widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.
The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix material. The...
282
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

733
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
733

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editorial: Navigating the microbial landscape: integrating mass spectrometry imaging and other multimodal imaging approaches for spatially resolved microbial studies.

Frontiers in cellular and infection microbiology·2026
Same author

Engineering <i>Pichia pastoris</i> as a Heterologous Platform for Non-Ribosomal Peptide Biosynthesis of the β‑Lactam Intermediate δ‑(l‑α-Aminoadipyl)‑l‑cysteinyl‑d‑valine (ACV).

ACS omega·2026
Same author

Influence of titer, rate, yield, and scale on the cost and life-cycle emissions of biomanufacturing.

Trends in biotechnology·2026
Same author

Influence of Acceleration Field and Inlet Temperature on Ion Yields of Small Molecules in Atmospheric Pressure MALDI.

International journal of mass spectrometry·2026
Same author

Precision integrated identification of predictive first-trimester metabolomics signatures for early detection of gestational diabetes mellitus.

Cardiovascular diabetology·2025
Same author

Insights on the spatial distribution of the <i>Pseudomonas aeruginosa</i> secondary metabolites under swarming motility-inducing conditions using mass spectrometry imaging.

Microbiology spectrum·2025

Related Experiment Video

Updated: Jun 12, 2025

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

12.7K

Direct Colony Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Assay for Targeted

Nivedita Bhattacharya1,2,3, Deepanwita Banerjee4, Caleigh O'Connor5

  • 1MassTech Inc., Columbia, MD, USA. nivedita@apmaldi.com.

Methods in Molecular Biology (Clifton, N.J.)
|June 11, 2025
PubMed
Summary

This study presents a rapid mass spectrometry method for analyzing bacterial metabolites like violacein with minimal sample prep. This technique enables efficient molecular phenotyping of bacterial colonies.

Keywords:
AP/MALDI MSAdaptive laboratory evolution (ALE)Antibiotic resistanceChromobacterium violaceumMetabolomicsMicrobial analysisPathway metabolitesVio operon

More Related Videos

A Tandem Liquid Chromatography&#8211;Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
08:03

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

Published on: March 28, 2017

10.1K
Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
09:49

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Published on: November 18, 2022

2.0K

Related Experiment Videos

Last Updated: Jun 12, 2025

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
07:34

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

12.7K
A Tandem Liquid Chromatography&#8211;Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
08:03

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

Published on: March 28, 2017

10.1K
Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
09:49

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Published on: November 18, 2022

2.0K

Area of Science:

  • Metabolomics
  • Microbiology
  • Analytical Chemistry

Background:

  • Bacterial metabolites are crucial for understanding microbial function.
  • Analyzing these compounds often requires extensive sample preparation.
  • Violacein, a pigment from Chromobacterium violaceum, serves as a model compound.

Purpose of the Study:

  • To develop a high-throughput, high-resolution mass spectrometry protocol.
  • To enable direct analysis of bacterial metabolites from colonies.
  • To facilitate targeted metabolite profiling of violacein and its intermediates.

Main Methods:

  • Atmospheric pressure matrix-assisted laser desorption/ionization (AP/MALDI) mass spectrometry (MS).
  • High-resolution (HR) MS for sensitive detection and quantification.
  • Direct analysis from bacterial colonies with minimal sample preparation.

Main Results:

  • Demonstrated sensitive and specific detection of violacein and intermediates.
  • Achieved relative quantification of metabolites.
  • Established a protocol suitable for high-throughput analysis.

Conclusions:

  • AP/MALDI MS offers a streamlined approach for bacterial metabolite analysis.
  • The developed protocol is effective for molecular phenotyping.
  • This method is applicable to various operon-controlled metabolites.