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Updated: May 10, 2026

Dissection of Human Vitreous Body Elements for Proteomic Analysis
Published on: January 23, 2011
LC-MS-Based Metabolic Fingerprinting of Vitreous Humor
Karolina Pietrowska1, Diana Anna Dmuchowska2, Joanna Godzien1
1Metabolomics and Proteomics Laboratory, Clinical Research Centre, Medical University of Bialystok, Bialystok, Poland.
Abstract:
Vitreous humor (VH) is the largest structure in the eye that fills the space between the lens and the retina. Its composition and level of metabolites may reflect the physiological and pathophysiological processes occurring in the retina. A protocol for the preparation of VH samples for untargeted metabolomics was developed, and extracted metabolites were analyzed using liquid chromatography-quadrupole-time of flight-mass spectrometry (LC-QTOF-MS). Two types of chromatography were combined with mass spectrometry, reversed-phase (RP-LC-MS), and hydrophilic interaction (HILIC-LC-MS).
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