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Updated: Jul 19, 2026

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
Published on: May 22, 2017
Revealing the Metabolome: Metabolite Workflow for Retinal Ganglion Cells
Alejandra Hernandez1,2, Sofia Yarosh1,2, Amr Almobayed1,2
1Bascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, USA.
Abstract:
In this chapter, we outline the preparation and isolation of retinal ganglion cells (RGCs) from postnatal mice. This includes a metabolite extraction method using 1:1 methanol/water from 6 or 12-well plate dishes. We provide liquid chromatography-mass spectrometry (LC-MS) parameters using a QExactive High Resolution Orbitrap Mass Spectrometer and HILIC column. The software used for analysis includes Compound Discoverer and MetaboAnalyst. Through this metabolic workflow, this protocol provides a biomarker discovery, allowing for future experimentation to treat and cure ocular neuropathies.

