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Growth and Polarization of A549 Human Alveolar Epithelial Cells as a Model to Study Biofilm Formation in

Luis A Ramos1, Elizabeth C Walls1, Mia S Petrucci1

  • 1Department of Biological Sciences, Florida Gulf Coast University, Fort Myers, FL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 11, 2025
PubMed

Keywords:
A549 human alveolar epithelial cellsAcinetobacterBiofilm formationCell polarizationRespiratory infection model

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Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...