Identification of the AKCDPK gene family and AkCDPK15 functional analysis under drought and salt stress
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View abstract on PubMed
Summary
This summary is machine-generated.Researchers identified 29 Calcium-Dependent Protein Kinase (CDPK) genes in konjac, with AkCDPK15 enhancing salt and drought tolerance in tobacco. This study provides insights into konjac
Area Of Science
- Plant Molecular Biology
- Genomics
- Stress Physiology
Background
- Konjac (Amorphophallus konjac) is a vital economic crop facing significant biotic and abiotic stresses.
- Calcium-dependent protein kinases (CDPKs) are crucial for plant stress responses, but their role in konjac is unknown.
Purpose Of The Study
- To identify and characterize the AkCDPK gene family in Amorphophallus konjac.
- To investigate the evolutionary patterns and stress-responsive functions of AkCDPK genes.
- To evaluate the potential of AkCDPK15 in enhancing stress tolerance.
Main Methods
- Genome-wide identification of AkCDPK genes using bioinformatics tools.
- Phylogenetic analysis and cis-acting element prediction.
- Gene expression profiling under various stress conditions (salt, drought, pathogen).
- Cloning, subcellular localization, and functional validation of AkCDPK15 in transgenic tobacco.
Main Results
- 29 AkCDPK genes were identified, distributed across 12 chromosomes and classified into four subgroups.
- Most AkCDPK genes showed evidence of purifying selection.
- Expression analysis revealed tissue specificity and differential regulation by salt, drought, and P. carotovorum stress.
- Overexpression of AkCDPK15 conferred enhanced tolerance to salt and drought stress in tobacco.
Conclusions
- The AkCDPK gene family plays a significant role in konjac's response to environmental stresses.
- AkCDPK15 is a key regulator of salt and drought tolerance.
- Findings lay the groundwork for developing stress-resilient konjac varieties through genetic improvement.
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