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Sustained in-ovo and in-feed probiotic supplementation promotes embryo development and post-hatch performance in
Yuying Ren1, Muhammed Shafeekh Muyyarikkandy1, Mairui Gao1
1Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
Poultry Science
|June 11, 2025
Summary
Supplementing broiler chickens with probiotics during embryonic development and post-hatch significantly improved growth, hatchability, and feed conversion ratio. This early and sustained probiotic approach enhances poultry production efficiency and bird health.
Area of Science:
- Poultry Science
- Animal Nutrition
- Microbiology
Background:
- Current broiler production focuses on post-hatch interventions, neglecting the critical embryonic phase.
- Embryonic development and early post-hatch stages significantly impact broiler performance.
- Probiotics offer a potential strategy to improve early development and growth.
Purpose of the Study:
- To evaluate the efficacy of probiotics in enhancing embryonic development and post-hatch performance in broiler chickens.
- To assess the impact of early and sustained probiotic supplementation on broiler growth and health.
Main Methods:
- Ross 308 broiler hatching eggs were treated with probiotics (Lactobacillus paracasei DUP 13076 and L. rhamnosus NRRL B 442) or a control solution.
- Embryonic development was assessed through morphometric analysis.
- Post-hatch chicks were raised for six weeks on probiotic-supplemented feed, with performance metrics evaluated.
Main Results:
- Probiotic treatment significantly improved embryonic development, hatchability, and hatchling weight.
- Sustained probiotic supplementation increased body weight gain, carcass weight, and relative breast/leg weight.
- Feed conversion ratio (FCR) was reduced, alongside improved performance efficiency and survivability.
Conclusions:
- Early and sustained probiotic supplementation enhances broiler growth and performance.
- Integrating probiotics during embryonic and post-hatch stages is a promising strategy for poultry production.
- Probiotics improve broiler efficiency and health under commercial conditions.

