Related Experiment Video
Updated: Jun 13, 2025
Fluorescent Paper Strips for the Detection of Diesel Adulteration with Smartphone Read-out
Published on: November 9, 2018
Smartphone-assisted ultrasensitive colorimetric strategy based on Cu-CN single-atom nanozyme for acrylamide detection
Jia Ge1, Xiaoyu Sun2, Xinxin Li2
1College of Chemistry, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou University, Zhengzhou 450001, China; School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
Abstract:
Herein, a novel colorimetric strategy based on Cu-CN single-atom nanozymes (Cu-CN SANs) is constructed for acrylamide detection. Cu-CN SANs exhibit excellent oxidase mimetic activity, which can efficiently catalyze the oxidation of substrate TMB to produce a blue product. GSH inhibits the oxidation of TMB by Cu-CN SANs. However, the addition of acrylamide enables the efficient thiolene Michael addition reaction between GSH and acrylamide, thus restoring the oxidation of TMB by Cu-CN SANs. The developed method enables acrylamide detection within a linear range of 0.05-15 μM, with a low detection limit (LOD) of 15.4 nM and a relative standard deviation (RSD%) below 4.3 %. When applied to real samples, the biosensor achieved satisfactory recoveries of 95.3-103.6 % for acrylamide analysis in bread, cookies, and chips samples. Additionally, integrating the sensor with a smartphone enabled on-site detection, yielding an LOD of 146 nM.
