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Related Concept Videos

Antibiotic Selection00:57

Antibiotic Selection

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Overview
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  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Medical Microbiology
  9. Medical Infection Agents (incl. Prions)
  11. Investigation Of Genomic Islands Involved In Antimicrobial Resistance In Proteus Mirabilis Isolated From Swine

Investigation of Genomic Islands Involved in Antimicrobial Resistance in Proteus mirabilis Isolated from Swine

Hui Su1, Lirong Yang1, Mimi Liu1

  • 1College of Animal Science and Technology, Jilin Agricultural Science and Technology University, 77 Hanlin Road, Jilin, 132101, Jilin, China.

Journal of Global Antimicrobial Resistance
|June 11, 2025

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Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
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Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis
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View abstract on PubMed

Summary
This summary is machine-generated.

Proteus mirabilis strain SH-4, isolated from swine, exhibits resistance to eleven antibiotics. Genomic analysis revealed mobile genetic elements like transposons and genomic islands that likely facilitate the spread of antibiotic resistance genes (ARGs).

Area of Science:

  • Microbiology
  • Genomics
  • Molecular Biology

Background:

  • Proteus mirabilis is a common bacterium found in natural environments and host intestines.
  • Antibiotic resistance in P. mirabilis poses significant challenges for treating infections.

Purpose of the Study:

  • To investigate the antibiotic resistance genes (ARGs) and genetic structure of P. mirabilis strain SH-4 isolated from a swine sample.
  • To understand the role of mobile genetic elements in the dissemination of ARGs within P. mirabilis.

Main Methods:

  • Antimicrobial susceptibility testing using the broth microdilution method.
  • Whole genome sequencing (WGS) and phylogenomic analysis of P. mirabilis SH-4.
  • Single nucleotide polymorphism (SNP) profiling to determine genetic relationships.
Keywords:
Antibiotic resistanceGenomic islandProteus mirabilisType IV secretion system

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Main Results:

  • P. mirabilis SH-4 possesses a 4,123,479 bp chromosome with 39.06% GC content.
  • The strain showed resistance to eleven tested antimicrobial agents.
  • WGS identified a ΔTn7 transposon and two genomic islands (PmGRI-9-SH-4 and PmGRI-10-SH-4), with the latter containing multiple Type IV secretion system (T4SS) genes and a multidrug resistance (MDR) region.

Conclusions:

  • Genomic islands (GIs) and insertion sequences (ISs) are key facilitators for acquiring antibiotic resistance genes in P. mirabilis.
  • These genetic elements contribute to the emergence of highly resistant P. mirabilis strains, complicating infection treatment.