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Proteomic Profiling Uncovers Sexual Dimorphism In The Muscle Response To Wheel Running Exercise In The Flexdux4 Murine Model Of Facioscapulohumeral Muscular Dystrophy.

Home
Research Domains
Engineering
Mechanical Engineering
Numerical Modelling And Mechanical Characterisation
Proteomic Profiling Uncovers Sexual Dimorphism In The Muscle Response To Wheel Running Exercise In The Flexdux4 Murine Model Of Facioscapulohumeral Muscular Dystrophy.

Related Experiment Video

Skeletal Muscle Gender Dimorphism from Proteomics

Skeletal Muscle Gender Dimorphism from Proteomics

Published on: December 14, 2011

Proteomic Profiling Uncovers Sexual Dimorphism in the Muscle Response to Wheel Running Exercise in the FLExDUX4 Murine Model of Facioscapulohumeral Muscular Dystrophy.

Yusuke Nishimura¹, Adam Bittel², Abhishek Jagan¹

¹Research Institute for Sport & Exercise Sciences, Liverpool John Moores University, Liverpool, United Kingdom.

Molecular & Cellular Proteomics : MCP

|June 11, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

Keywords:

DUX4 FSHD RNA-binding proteins apoptosis

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Published on: January 31, 2013

Facioscapulohumeral muscular dystrophy (FSHD) mouse models show altered muscle proteins, impacting mitochondria and RNA metabolism. Sex-specific differences in response to exercise highlight the need for tailored FSHD biomarkers.

Area of Science:

Biochemistry
Genetics
Musculoskeletal Biology

Background:

Facioscapulohumeral muscular dystrophy (FSHD) is a genetic disorder affecting skeletal muscles.
The FLExDUX4 mouse model mimics FSHD, exhibiting similar pathologies and functional deficits.
Proteomic analysis offers insights into FSHD's complex molecular mechanisms, particularly post-transcriptional regulation.

Purpose of the Study:

To characterize the triceps brachii proteome in FLExDUX4 mice compared to wild-type controls.
To investigate the impact of exercise (voluntary wheel running) on muscle proteomes in both genotypes.
To identify sex-specific proteomic alterations in the context of FSHD and exercise.

Main Methods:

Mass spectrometry-based proteomics was employed to quantify protein abundance.

oxidative stress

skeletal muscle

Triceps brachii muscles from male and female wild-type and FLExDUX4 mice were analyzed.

Mice underwent a 6-week free voluntary wheel running (VWR) protocol.

Main Results:

The FLExDUX4 mouse proteome reflects key FSHD skeletal muscle characteristics, including changes in mitochondria, RNA metabolism, oxidative stress, and apoptosis.
RNA-binding protein abundance showed sex-specific differences in FLExDUX4 mice.
Exercise induced sexual dimorphism in mitochondrial protein adaptation, with females increasing and males decreasing these proteins in FLExDUX4 mice.

Conclusions:

The FLExDUX4 mouse model accurately recapitulates aspects of FSHD muscle pathology.
Sex-specific differences in protein expression and response to exercise are significant in FSHD.
Identifying sex-specific biomarkers is crucial for effective FSHD monitoring and therapeutic development.