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Related Concept Videos

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions12:18

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This protocol describes the formation of cell mimicking uni-lipid and multi-lipid vesicles, supported lipid bilayers, and suspended lipid bilayers. These in vitro models can be adapted to incorporate a variety of lipid types and can be used to investigate various molecule and macromolecule interactions.
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Here, we present an experimental protocol that can be employed to determine the binding affinities and mode of interaction of label-free phospholipid-binding protein annexin A2 with immobilized solid-supported bilayers (SLB) by simultaneously measuring the mass uptake and the viscoelastic properties of the protein annexin...
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This video demonstrates a dissipative microgravimetry technique to investigate the binding of proteins to lipid bilayers. Small unilamellar vesicles are added to coat the surface of the quartz sensor of a microbalance. Utilizing the calcium ion-dependent binding of the target phospholipid-binding protein to the bilayer, changes in the oscillation frequency of the sensor and the dissipation of the oscillation are monitored to determine the nature of the protein-lipid bilayer...
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Quantitative Flow Cytometry to Study Labeled Protein-Phospholipid Vesicle Interactions02:43

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Related Experiment Video

Updated: Jan 20, 2026

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
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X-ray studies on phospholipid bilayers. V. Interactions with DDT.

M Suwalsky, N Bugueño, J Tapia

    Zeitschrift Fur Naturforschung. Section C, Biosciences
    |July 1, 1985
    PubMed
    Summary

    This study investigated DDT interactions with lipids like DML, DPPE, and TP using X-ray diffraction. No new phases formed, indicating no significant interactions between DDT and these lipids.

    Area of Science:

    • Lipid-drug interactions
    • X-ray crystallography
    • Chemical analysis

    Background:

    • Understanding the interaction of pesticides like DDT with biological lipids is crucial for assessing their environmental fate and toxicological effects.
    • Lipids such as dimyristoyl lecithin (DML), dipalmitoylphosphatidylethanolamine (DPPE), and tripalmitin (TP) are common components of biological membranes and tissues.

    Purpose of the Study:

    • To investigate the potential interactions between the insecticide DDT and specific lipids (DML, DPPE, TP).
    • To determine if new chemical phases or complexes form upon mixing DDT with these lipids.

    Main Methods:

    • X-ray diffraction analysis was employed on oriented films and crystalline powders of DDT-lipid mixtures.
    • Mixtures were prepared at various molar ratios to explore concentration-dependent effects.

    More Related Videos

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    03:36

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    Quantitative Flow Cytometry to Study Labeled Protein-Phospholipid Vesicle Interactions
    02:43

    Quantitative Flow Cytometry to Study Labeled Protein-Phospholipid Vesicle Interactions

    607

    Related Experiment Videos

    Last Updated: Jan 20, 2026

    Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
    07:11

    Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator

    Published on: November 1, 2018

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    Dissipative Microgravimetry Technique to Study Protein-Lipid Bilayer Interaction
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    Dissipative Microgravimetry Technique to Study Protein-Lipid Bilayer Interaction

    475
    Quantitative Flow Cytometry to Study Labeled Protein-Phospholipid Vesicle Interactions
    02:43

    Quantitative Flow Cytometry to Study Labeled Protein-Phospholipid Vesicle Interactions

    607
  • Diffraction patterns of pure DDT and pure lipids were used as controls.

    • Main Results:

    • X-ray diffraction patterns of the DDT-lipid mixtures exclusively displayed the characteristic patterns of pure DDT and the respective pure lipid.
    • No new or altered diffraction peaks were observed, which would indicate the formation of novel phases or complexes.
    • The absence of new phases suggests a lack of specific molecular interactions between DDT and the studied lipids.

    Conclusions:

    • DDT does not form new phases or exhibit significant interactions with dimyristoyl lecithin, dipalmitoylphosphatidylethanolamine, or tripalmitin under the studied conditions.
    • The physical state of DDT and these lipids remains independent when mixed, suggesting no chemical complexation or intercalation.
    • These findings contribute to understanding the behavior of DDT in lipid-rich environments.