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Solution conformational differences between conventional and CENP-A nucleosomes are accentuated by reversible

Kushol Gupta1, Nikolina Sekulić2,3,4, Praveen Kumar Allu5

  • 1Department of Biochemistry & Biophysics, Perelman School of Medicine, University of Pennsylvania, 422 Curie Boulevard, Philadelphia, PA, 19104-6059, USA. kgupta@pennmedicine.upenn.edu.

Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology
|June 11, 2025
PubMed
Summary

We compared standard nucleosomes with centromeric nucleosomes using scattering techniques. Histone and DNA sequences impact nucleosome stability under pressure, with centromeric nucleosomes showing greater resilience.

Keywords:
Analytical ultracentrifugationCentromeresHigh-pressureNucleosomeSmall-angle X-ray scatteringSmall-angle neutron scattering

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