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Updated: Jun 13, 2025
Pre-Chiasmatic, Single Injection of Autologous Blood to Induce Experimental Subarachnoid Hemorrhage in a Rat Model
Published on: June 18, 2021
Development and validation of modified prechiasmatic rat model of subarachnoid hemorrhage
1Department of Neurology, First Affiliated Hospital of Guangxi Medical University, Nanning City, China.
Background:
Subarachnoid hemorrhage (SAH) is a severe type of stroke characterized by high morbidity and mortality. Early brain injury (EBI) plays an important role in determining the prognosis of SAH. Currently, few animal models fully simulate the disease process, and the modeling procedures are complicated and error-prone. We aimed to develop a modified prechiasmatic injection rat model that is reproducible and easy to perform.
Materials And Methods:
Male Sprague-Dawley rats were randomly allocated to the sham group (n = 15) and the model group (n = 15). A burr hole was drilled in the skull, 1 mm beside the intersection of the midline and transverse line, and 30 μL of autologous blood was slowly injected into the prechiasmatic pool.
Results:
The mortality rate in the model group within 24 hours post-modeling was 13.33% (2/15). Blood clots were observed around the prechiasmatic pool and its periphery, indicating successful replication of SAH. Neurologic functions, brain water content, and Nissl staining confirmed the pathology of early brain injury. GPX4, GSH, MDA, and iron levels indicated the presence of ferroptosis.
Conclusions:
The proposed modified prechiasmatic injection rat model is feasible and reproducible. The model has the potential to be used for investigating mechanisms of subarachnoid hemorrhage and early brain injury.
