Abstract
OBJECTIVE
To develop a highly sensitive and specific detection method based on PCR-CRISPR/Cas12a for the detection of Streptococcus anginosus (S. anginosus) in feces and to evaluate its detection rate in the general population as well as its potential as a gastrointestinal tumor marker.
MATERIALS AND METHODS
Specific primers and crRNA targeting the 16S rDNA of S. anginosus were designed to construct a PCR-CRISPR/Cas12a detection system. A total of 230 fecal samples were collected from the general population, and bacterial DNA was extracted. The target gene was detected using this system to verify its sensitivity, specificity, and stability.
RESULTS
The established detection system demonstrated strong specificity, with stable recognition of S. anginosus, and a minimum detection limit of 10-7 ng/μL. The detection rate of S. anginosus in fecal samples from the general population was 51.7%.
CONCLUSION
The PCR-CRISPR/Cas12a system can efficiently detect S. anginosus in feces, providing a reliable technical tool for exploring its association with gastrointestinal tumors.