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Detection of Streptococcus anginosus in fecal samples using PCR-CRISPR /Cas12a system
Yan Zheng1, BiXia Liu2, QianFei Zuo3
1Department of Gastroenterology, Chongqing Key Laboratory of Digestive Malignancies, Daping Hospital, Army Medical University (Third Military Medical University), Chongqing, PR China.
Objective:
To develop a highly sensitive and specific detection method based on PCR-CRISPR/Cas12a for the detection of Streptococcus anginosus (S. anginosus) in feces and to evaluate its detection rate in the general population as well as its potential as a gastrointestinal tumor marker.
Materials And Methods:
Specific primers and crRNA targeting the 16S rDNA of S. anginosus were designed to construct a PCR-CRISPR/Cas12a detection system. A total of 230 fecal samples were collected from the general population, and bacterial DNA was extracted. The target gene was detected using this system to verify its sensitivity, specificity, and stability.
Results:
The established detection system demonstrated strong specificity, with stable recognition of S. anginosus, and a minimum detection limit of 10-7 ng/μL. The detection rate of S. anginosus in fecal samples from the general population was 51.7%.
Conclusion:
The PCR-CRISPR/Cas12a system can efficiently detect S. anginosus in feces, providing a reliable technical tool for exploring its association with gastrointestinal tumors.
