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Updated: Jun 13, 2025

A High-content Assay for Monitoring AMPA Receptor Trafficking
Published on: January 28, 2019
Rab10 inactivation promotes AMPAR trafficking and spine enlargement during long-term potentiation
Jie Wang1,2,3, Jun Nishiyama2,4, Paula Parra-Bueno2
1Department of Neurobiology, Duke University School of Medicine, Durham, NC, 27710, USA.
Abstract:
Rab-dependent membrane trafficking is critical for changing the structure and function of dendritic spines during synaptic plasticity. Here, we developed highly sensitive sensors to monitor Rab protein activity in single dendritic spines undergoing structural long-term potentiation (sLTP) in rodent organotypic hippocampal slices. During sLTP, Rab10 was persistently inactivated (>30 min) in the stimulated spines, whereas Rab4 was transiently activated over ~5 min. Inhibiting or deleting Rab10 enhanced sLTP, electrophysiological LTP and AMPA receptor (AMPAR) trafficking during sLTP. In contrast, disrupting Rab4 impaired sLTP only in the first few minutes, and decreased AMPAR trafficking during sLTP. Thus, our results suggest that Rab10 and Rab4 oppositely regulate AMPAR trafficking during sLTP, and inactivation of Rab10 signaling facilitates the induction of LTP and associated spine structural plasticity.
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