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The Sphingosine-1-phosphate pathway is differentially activated in human gestational tissues.

Magdaleena Naemi Mbadhi1, Hideji Fujiwara2, Ruth Gill1

  • 1Center for Reproductive Health Sciences, Department of Obstetrics and Gynecology, Washington University School of Medicine, St. Louis, MO 63110, USA.

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PubMed
Summary
This summary is machine-generated.

Sphingosine-1-phosphate (S1P) metabolism and enzymes are regulated differently in pregnancy tissues. Increased S1P in the myometrium during labor suggests a role in myometrial contractility.

Keywords:
Decidua parietalisS1Pmetabolomicsmyometriumpregnancypreterm laborsphingolipidsterm labor

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Area of Science:

  • Obstetrics and Gynecology
  • Biochemistry
  • Reproductive Biology

Background:

  • Dysregulated myometrial contractility is linked to obstetric complications.
  • Sphingosine-1-phosphate (S1P) is an inflammatory regulator in pregnancy tissues, but its metabolic dynamics are unclear.
  • Understanding S1P metabolism is crucial for addressing pregnancy complications.

Purpose of the Study:

  • To profile Sphingosine-1-phosphate (S1P) metabolic enzyme and receptor expression in human gestational tissues.
  • To quantify sphingolipid metabolism across different stages of pregnancy.
  • To investigate the role of S1P in myometrial contractility.

Main Methods:

  • Collected human myometrium, decidua parietalis, and chorioamnion from term without labor, term with labor, and preterm without labor pregnancies.
  • Assessed messenger RNA (mRNA) expression of S1P metabolic enzymes and receptors via quantitative polymerase chain reaction.
  • Quantified sphingolipids using targeted liquid chromatography-tandem mass spectrometry.

Main Results:

  • Sphingosine-1-phosphate (S1P) metabolic enzymes and receptors showed differential expression across gestational tissues.
  • Myometrium had higher S1P receptor expression and S1P abundance compared to decidua and chorioamnion at term.
  • Both S1P and SPHK1 expression increased significantly in the myometrium with labor compared to without labor.

Conclusions:

  • Sphingosine-1-phosphate (S1P) metabolism and signaling are distinctly regulated in human gestational tissues.
  • Findings suggest a potential therapeutic role for S1P in managing myometrial contractility.
  • Tissue-specific S1P regulation offers insights into obstetric complication mechanisms.