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Updated: Jun 14, 2025
LAD-Ligation: A Murine Model of Myocardial Infarction
Published on: October 14, 2009
Cell-free extracts from human fat tissue attenuate ischemic injury in cardiomyocytes in a murine model
Tian-Yun Yang1, Yi Sun1, Wen-Jie Zhang2
1Department of Cardiology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Background:
Ischemic heart disease ranks among the foremost contributors to mortality worldwide. Myocardial infarction injury poses a prevalent challenge in current therapies. Studies have shown that mesenchymal stem cell transplantation increases cytokine release, reduces myocardial cell necrosis, and improves left ventricular function; thus, it can be used to understand protective mechanisms. Fat extract (FE) derived from mesenchymal stem cell therapy contains high levels of paracrine factors.
Aim:
To study the effects of FE on myocardial injury and its mechanism of action.
Methods:
A mouse model of myocardial infarction and a hypoxic model of neonatal rat cardiomyocytes (CMs) were established to evaluate the effects of FE.
Results:
FE exhibited an inhibitory effect on CM apoptosis and improved left ventricular function. This protective effect of FE on CMs was mediated, in part, by the activation of the phosphatidylinositol 3-kinase/protein kinase B/mechanistic target of rapamycin signaling pathway.
Conclusion:
Our findings showed that FE could be a new treatment to protect CMs in ischemic heart disease.
