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Expanded PCR panel for uropathogen identification and treatment recommendations in urinary tract infections
Lindsey Leech1, Christopher Bigley1, Marshall Chew1
1Vikor Scientific, Charleston, SC, USA.
Therapeutic Advances in Urology
|June 12, 2025
Summary
Multiplex PCR accurately identifies more uropathogens than standard urine culture in complicated UTI cases. This advanced molecular test improves diagnosis and treatment, reducing patient morbidity.
Area of Science:
- Clinical microbiology
- Molecular diagnostics
- Infectious diseases
Background:
- Urinary tract infections (UTIs) are common and costly, posing diagnostic challenges.
- Standard urine culture (SUC) has limitations in pathogen detection and speed.
- Multiplex PCR offers rapid, simultaneous detection of uropathogens and antimicrobial resistance (AMR) genes.
Purpose of the Study:
- To compare the diagnostic performance of the Urine-ID™ multiplex PCR test against SUC.
- To evaluate pathogen detection in individuals with suspected complicated UTIs.
Main Methods:
- Retrospective analysis of 56 urine specimens from patients aged 50+ with suspected complicated UTIs.
- Urine-ID™ multiplex PCR was performed on TaqMan® OpenArray plates using QuantStudio 12K Flex Real-Time PCR System.
- Results were compared with simultaneously collected SUC at patient follow-ups.
Main Results:
- SUC failed to detect pathogens in 19.64% of cases, while PCR was negative in 7.14%.
- PCR detected uropathogens in 92.86% of specimens, compared to 50% by SUC.
- PCR identified additional co-infecting pathogens in 71.20% of positive samples, which SUC missed.
Conclusions:
- Expanded real-time PCR panels like Urine-ID™ significantly surpass SUC sensitivity for uropathogen identification.
- These advanced molecular techniques improve diagnostic efficiency and speed for complicated UTIs.
- Faster pathogen identification leads to timely, targeted therapy, reducing patient morbidity.
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