Related Experiment Video
Updated: May 9, 2026
Micropatterned Surfaces to Study Hyaluronic Acid Interactions with Cancer Cells
Published on: December 22, 2010
Self-Assembly of Alkynylplatinum(II) Complexes for Sialic Acid Detection and Differentiation of Cancer Cells from
Jungu Guo1,2, Eric Ka-Ho Wong1, Guang-Xi Xu3
1Institute of Molecular Functional Materials and Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong 999077, People's Republic of China.
Abstract:
A series of platinum(II) complexes incorporating a histidine moiety and/or positively charged group has been designed and synthesized to explore the specific binding between platinum(II) complexes and the analyte and their sensing ability. The specific hydrogen bonding between the histidine moiety and sialic acids, and the electrostatic interaction between the positively charged trimethylammonium group of the platinum(II) complexes and negatively charged carboxylate groups of sialic acids have been found to enhance the binding affinity. The supramolecular assembly of platinum(II) complexes upon binding to sialic acids induces remarkable luminescence changes due to noncovalent Pt(II)···Pt(II) and π-π stacking interactions, achieving sensing and visualization of sialic acids. A novel luminescence assay has been developed to differentiate cancer cells from normal cells based on the supramolecular assembly of platinum(II) complexes upon binding to a high level of sialic acids due to its excellent imaging ability toward sialic acids in live cells. The complexes have been adopted to visualize the variation of sialic acids in live cells after treatment with enzymes to remove sialic acids, paving the way for screening of novel anticancer agents. The present sensing strategy for differentiating cancer cells from normal cells facilitates early diagnosis and therapeutic guidance.
More Related Videos
07:20Amide Coupling Reaction for the Synthesis of Bispyridine-based Ligands and Their Complexation to Platinum as Dinuclear Anticancer Agents
Published on: May 28, 2014
13:36Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface
Published on: October 20, 2023
Related Concept Videos
02:37Structure and Physical Properties of Alkynes
In nature, compounds containing both carbon and hydrogen are known as "hydrocarbons". Aliphatic hydrocarbons are compounds whose molecules contain saturated single bonds (i.e., alkanes) or unsaturated double or triple bonds. Alkenes contain carbon–carbon double bonds and have a structural formula CnH2n. Unsaturated hydrocarbons containing carbon–carbon triple bonds are called "alkynes" and are structurally represented by the formula CnH2n-2.
The simplest alkyne is ethyne, or...
02:27Preparation of Alkynes: Alkylation Reaction
Alkylation of terminal alkynes with primary alkyl halides in the presence of a strong base like sodium amide is one of the common methods for the synthesis of longer carbon-chain alkynes. For example, treatment of 1-propyne with sodium amide followed by reaction with ethyl bromide yields 2-pentyne.
02:34Preparation of Alkynes: Dehydrohalogenation
Alkynes can be prepared by dehydrohalogenation of vicinal or geminal dihalides in the presence of a strong base like sodium amide in liquid ammonia. The reaction proceeds with the loss of two equivalents of hydrogen halide (HX) via two successive E2 elimination reactions.
02:38Electrophilic Addition to Alkynes: Halogenation
Halogenation is another class of electrophilic addition reactions where a halogen molecule gets added across a π bond. In alkynes, the presence of two π bonds allows for the addition of two equivalents of halogens (bromine or chlorine). The addition of the first halogen molecule forms a trans-dihaloalkene as the major product and the cis isomer as the minor product. Subsequent addition of the second equivalent yields the tetrahalide.
02:35Electrophilic Addition to Alkynes: Hydrohalogenation
02:24Reduction of Alkynes to cis-Alkenes: Catalytic Hydrogenation
Like alkenes, alkynes can be reduced to alkanes in the presence of transition metal catalysts such as Pt, Pd, or Ni. The reaction involves two sequential syn additions of hydrogen via a cis-alkene intermediate.