Research Progress on bioactive peptides from animal sources: A comprehensive review

Area of Science:

• Biochemistry
• Food Science
• Pharmacology

Background:

• Bioactive peptides from animals possess significant antitumor, antibacterial, and antioxidant properties.
• Preclinical studies indicate their potential as functional foods for disease management.
• Established research provides a foundation for optimizing peptide preparation and purification.

Purpose of the Study:

• To review optimized methods for enzymatic hydrolysis to improve bioactive peptide yield and bioactivity.
• To highlight advanced separation techniques for efficient purification of bioactive peptides.
• To discuss mass spectrometry applications for characterizing bioactive peptides, their mechanisms, structure-activity relationships, and in vivo metabolism.

Main Methods:

• Enzymatic hydrolysis optimization for enhanced peptide yield and bioactivity.
• Combination separation techniques for efficient bioactive peptide purification.
• Mass spectrometry for molecular weight determination and structural analysis.

Main Results:

• Optimized hydrolysis and separation methods significantly improve bioactive peptide yield and purity.
• Mass spectrometry enables detailed characterization of peptide properties and functions.
• Understanding in vivo metabolism is crucial for therapeutic development.

Conclusions:

• Animal-derived bioactive peptides offer therapeutic potential for various health conditions.
• Advanced preparation, purification, and analytical techniques are key to unlocking this potential.
• Further research into their mechanisms and metabolism will facilitate their development into health and functional foods.