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Updated: Jun 14, 2025

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
Published on: December 18, 2019
AARS1-mediated Osterix lactylation promotes its transcriptional activity during osteoblast differentiation
Feige Nian1, Linfeng Guo2, Zhangli Fei1
1Department of Rheumatology and immunology, The Affiliated Hospital of Jiaxing University, The First Hospital of Jiaxing, No. 1882 Zhonghuan South Road, Jiaxing, Zhejiang 314000, China.
Abstract:
AARS1 is a newly reported lactyl-transferase that plays vital roles in tumorigenesis and innate immune response. However, the function of AARS1-mediated lactylation in osteoblast differentiation is still unclear. Here, we found that silencing of AARS1 impaired the ALP activity and formation of mineralized nodules during osteoblast differentiation. Additionally, our findings demonstrated that AARS1 catalyzed lactylation of Osterix (Osx), a crucial transcription factor involved in the differentiation process of osteoblast cells. Lactylation of Osx increased its binding to target genes and promoted the interaction between Osx and WDR5, facilitating H3K4 tri-methylation on downstream target genes. This in turn enhanced the expression of Osx target genes and osteoblast differentiation. In summary, our study revealed a novel role of AARS1-mediated Osx lactylation during osteoblast differentiation.
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