Abstract
BACKGROUND
Although donor lymphocyte infusion (DLI) is a treatment option after posttransplant immunomodulation, data on donor lymphocyte apheresis (DLA) remain scarce.
METHODS
We retrospectively analyzed 26 recipients receiving DLI and their related donors (human leukocyte antigen [HLA]-matched, n = 9; haploidentical, n = 17).
RESULTS
The median harvested CD3-positive cell count (CD3) was 6.71 × 107 cells/kg (range, 3.55-19.74). A positive correlation was observed between harvested CD3 and preharvest lymphocyte counts (correlation coefficients = 0.78). In HLA-matched DLI, the median infused CD3 was 1.19 × 107 per cycle and 4.56 × 107 cells/kg in total; in haploidentical DLI, 0.25 × 107 and 0.44 × 107, respectively. The proportion of total infused CD3 relative to harvested CD3 was 26.3% (range, 1.4-100) in HLA-matched DLI and 4.5% (range, 1.4-100) in HLA-haploidentical DLI.
CONCLUSIONS
Our findings reflect real-world practices and underscore the importance of tailoring DLA to donor and recipient characteristics, particularly in light of the increasing use of haploidentical donors.
