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  3. Research Domains
  5. Agricultural, Veterinary And Food Sciences
  7. Horticultural Production
  9. Horticultural Crop Protection (incl. Pests, Diseases And Weeds)
  11. Integrative Transcriptome And Metabolome Analyses Provide Insights Into Rice Defence Against Chilo Suppressalis Oviposition

Integrative transcriptome and metabolome analyses provide insights into rice defence against Chilo suppressalis oviposition

Chen Shen1,2, Haibo Bao1,3, Zhengping Yu1,2

  • 1Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Food and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, China.

Plant Molecular Biology
|June 12, 2025

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View abstract on PubMed

Summary
This summary is machine-generated.

Rice plants mount molecular defenses against the striped stem borer (Chilo suppressalis) egg-laying. This study reveals gene expression and metabolite changes, and deterred oviposition behavior in response to insect eggs.

Area of Science:

  • Plant Science
  • Entomology
  • Molecular Biology

Background:

  • Rice (Oryza sativa) is a vital global food crop facing yield losses from pests like the striped stem borer (Chilo suppressalis).
  • While rice responses to larval feeding are known, molecular and biochemical reactions to C. suppressalis oviposition are less understood.
  • Insect egg deposition often primes plant defense mechanisms.

Purpose of the Study:

  • To investigate the molecular and biochemical responses of rice to C. suppressalis oviposition.
  • To analyze changes in gene expression and metabolite profiles in rice leaves after egg-laying.
  • To assess the impact of oviposition on C. suppressalis behavior.

Main Methods:

  • Next-generation RNA sequencing to analyze gene expression.
  • Comprehensive metabolomics to profile changes in metabolites.
Keywords:
Chilo suppressalisOryza sativadefence responseoviposition

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  • Oviposition behavioral assays with C. suppressalis on rice plants.

    • Main Results:

    • 1,350 differentially expressed genes and 234 differential metabolites were identified 24 hours post-oviposition.
    • Upregulated genes were associated with plant defense, stress responses, and secondary metabolism.
    • Increased levels of lipids, flavonoids, terpenoids, and phenolic compounds were observed, similar to pathogen responses. Oviposition was deterred on egg-laden rice.

    Conclusions:

    • Rice exhibits induced molecular and biochemical defense mechanisms against C. suppressalis oviposition.
    • Findings provide insights into plant-insect interactions and potential strategies for pest resistance.
    • Results may inform the development of ovicidal agents and insect-resistant rice varieties.