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Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

4.2K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.2K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

7.3K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.3K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

8.7K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.7K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

7.3K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.3K
Yeast Signaling01:28

Yeast Signaling

14.5K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
14.5K

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Related Experiment Video

Updated: Jun 14, 2025

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
09:08

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

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Role of Estrogen Signaling in Notch Pathway Activation in Sertoli Cells.

Sylwia Lustofin1, Alicja Kamińska1, Barbara Bilińska1

  • 1Department of Endocrinology, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, Gronostajowa 9, Krakow, 30-387, Poland.

Reproductive Sciences (Thousand Oaks, Calif.)
|June 12, 2025
PubMed
Summary

Estrogen signaling, particularly via estrogen receptor 2, enhances Notch1 pathway activity in Sertoli cells, crucial for spermatogenesis. This reveals a new mechanism for estrogen in maintaining testicular homeostasis.

Keywords:
17β-estradiolESR1ESR2GPER1Notch pathwaySertoli cellTestis

More Related Videos

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
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Stimulation of Notch Signaling in Mouse Osteoclast Precursors

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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Published on: January 2, 2018

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Related Experiment Videos

Last Updated: Jun 14, 2025

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
09:08

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

6.7K
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
05:48

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Published on: January 2, 2018

7.5K

Area of Science:

  • Reproductive Biology
  • Endocrinology
  • Cell Signaling

Background:

  • Sertoli cells are vital for spermatogenesis in mammalian testes.
  • These cells respond to androgens and estrogens, key reproductive hormones.
  • Estrogen signaling's role in Sertoli cell function, specifically the Notch pathway, requires further elucidation.

Purpose of the Study:

  • To investigate the impact of estrogen signaling on the canonical Notch pathway in rodent Sertoli cells.
  • To determine the specific estrogen receptors involved in this interaction.
  • To understand how estrogen influences cell-to-cell communication within the seminiferous epithelium.

Main Methods:

  • Utilized a Sertoli cell line and primary Sertoli cell cultures.
  • Administered 17β-estradiol to assess its effects on Notch pathway components.
  • Measured Notch1 activation, RBPJ transcriptional activity, and effector gene/protein expression (Hes1, Hey1).

Main Results:

  • 17β-estradiol, via estrogen receptor 2, boosted Notch1 activation and Hes1 expression.
  • 17β-estradiol binding to estrogen receptor 1 decreased Hey1 gene expression.
  • G protein-coupled estrogen receptor 1 showed no significant role in regulating Notch1 signaling.

Conclusions:

  • Nuclear estrogen receptors play a key role in controlling Notch1 pathway activity in Sertoli cells.
  • Estrogen receptor 2-mediated signaling enhances Notch1 activation and Hes1 expression.
  • Estrogen receptor 1-mediated signaling suppresses Hey1 expression, indicating differential regulation.
  • This study uncovers a novel mechanism of estrogen action in maintaining seminiferous epithelium homeostasis.