Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Digestion01:02

Protein Digestion

Protein digestion begins in the stomach, where the highly acidic environment can easily disrupt protein structure by exposing the peptide bonds of polypeptide chains. After polypeptide chains are broken into individual amino acids by a series of digestive enzymes, the amino acids are transported to the liver via the bloodstream to produce energy.
Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Proteins: Dietary Sources and Requirements01:28

Proteins: Dietary Sources and Requirements

Consuming animal-based products offers high-quality proteins that contain optimal levels and combinations of essential amino acids, crucial for tissue repair and growth. Foods like eggs, milk, fish, and most meats are a source of complete proteins. Legumes and cereals are abundant in proteins; however, they typically lack a full range of essential amino acids. As a result, they are considered incomplete protein sources. Some plant sources like soybeans, quinoa, and amaranth do contain complete...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improving the protein quality of New Zealand vegan diets: an optimisation modelling approach incorporating energy constraints and diet acceptability.

Frontiers in nutrition·2026
Same author

Development of the Vegan Protein Quality (VPQ) tool to derive smarter vegan meals with high protein quality.

Scientific reports·2026
Same author

Beyond daily totals: meal-level digestible indispensable amino acid score reveals how food groups shape protein quality in vegan diets.

Frontiers in nutrition·2026
Same author

Normalization of Gastrointestinal Symptoms in Adults With Constipation With Daily Green Kiwifruit Consumption: Protocol for an Open-Label Intervention Study.

JMIR research protocols·2026
Same author

Integrated multi-omic and symptom clustering reveals lower-gastrointestinal disorders of gut-brain interaction heterogeneity.

Gut microbes·2025
Same author

Nutrient-adequate diets with the lowest greenhouse gas emissions or price are the least acceptable-insights from dietary optimisation modelling using the iOTA model<sup>®</sup>.

Frontiers in nutrition·2025

Related Experiment Video

Updated: Jun 9, 2026

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
12:47

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

Published on: January 22, 2018

9.4K

Protein Intake and Protein Quality Patterns in New Zealand Vegan Diets: An Observational Analysis Using Dynamic Time

Bi Xue Patricia Soh1, Matthieu Vignes1,2, Nick W Smith1

  • 1Sustainable Nutrition Initiative, Riddet Institute, Massey University, Palmerston North 4410, New Zealand.

Nutrients
|June 13, 2025
PubMed
Summary

Vegan diets often lack sufficient indispensable amino acids (IAAs). This study used Dynamic Time Warping (DTW) to analyze protein and IAA intake patterns in New Zealand vegans, revealing suboptimal intake across most eating occasions.

Keywords:
amino acidsdata drivenhierarchical clusteringmeal patternprotein digestibilityprotein intakeprotein qualityvegan diets

More Related Videos

In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2
09:45

In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2

Published on: June 13, 2020

6.2K
Author Spotlight: Improving Beef Cattle Nutrition and Production with a Focus on Feed Efficiency and Meat Quality Traits Through Advanced Biochemical and Molecular Assays
07:46

Author Spotlight: Improving Beef Cattle Nutrition and Production with a Focus on Feed Efficiency and Meat Quality Traits Through Advanced Biochemical and Molecular Assays

Published on: July 12, 2024

484

Related Experiment Videos

Last Updated: Jun 9, 2026

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
12:47

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

Published on: January 22, 2018

9.4K
In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2
09:45

In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2

Published on: June 13, 2020

6.2K
Author Spotlight: Improving Beef Cattle Nutrition and Production with a Focus on Feed Efficiency and Meat Quality Traits Through Advanced Biochemical and Molecular Assays
07:46

Author Spotlight: Improving Beef Cattle Nutrition and Production with a Focus on Feed Efficiency and Meat Quality Traits Through Advanced Biochemical and Molecular Assays

Published on: July 12, 2024

484

Area of Science:

  • Nutritional Science
  • Dietary Analysis
  • Vegan Nutrition

Background:

  • Vegan diets pose challenges in meeting indispensable amino acid (IAA) requirements.
  • Consistent, balanced protein intake is crucial for metabolic health as IAAs are not stored by the body.
  • Optimizing protein and IAA intake is essential for individuals following vegan diets.

Purpose of the Study:

  • To investigate variations in 24-hour protein and IAA intake among New Zealand vegans.
  • To categorize dietary patterns using time-series clustering with Dynamic Time Warping (DTW).
  • To analyze protein intake and quality across different dietary clusters.

Main Methods:

  • Employed a data-driven approach using Dynamic Time Warping (DTW) for time-series clustering.
  • Objectively categorized vegan dietary data based on protein intake and quality.
  • Analyzed protein and IAA consumption patterns across distinct dietary clusters.

Main Results:

  • 93.5% of participants consumed less than the recommended 20g protein per eating occasion (EO).
  • Mean protein intake per EO varied significantly across clusters (6.5g, 11.4g, 19.0g).
  • Indispensable amino acid intake, particularly lysine and leucine, was substantially higher in the highest protein intake cluster (Cluster 3).

Conclusions:

  • Dynamic Time Warping (DTW) successfully identified three distinct dietary patterns within the vegan cohort.
  • The majority of eating occasions across all clusters fell below recommended protein intake thresholds.
  • Further research into plant-based protein combinations is recommended to enhance protein quality in vegan diets.