Cooked Bean (Phaseolus vulgaris L.) Consumption Alters Bile Acid Metabolism in a Mouse Model of Diet-Induced Metabolic Dysfunction: Proof-of-Concept Investigation
Tymofiy Lutsiv1,2, Vanessa K Fitzgerald1, Elizabeth S Neil1
1Cancer Prevention Laboratory, Colorado State University, Fort Collins, CO 80523, USA.
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View abstract on PubMed
Common bean consumption improves metabolic health by altering bile acid metabolism. This dietary change helps mice resist obesity and liver disease, offering potential benefits for dyslipidemia.
Area of Science:
- Metabolomics
- Microbiome research
- Nutritional science
Background:
- Metabolic dysregulation drives chronic diseases like obesity and MASLD.
- Bile acids are key mediators in metabolic disease development.
- Dietary interventions, including pulses like common beans, can improve metabolic health.
Purpose of the Study:
- To investigate how common bean consumption impacts bile acid metabolism.
- To assess the effects of common beans on metabolic dysfunction-associated steatotic liver disease (MASLD) and obesity.
Main Methods:
- Analysis of bile acids, metabolomics, and liver gene/protein expression in mice fed control or bean-containing diets.
- 16S rRNA and shotgun metagenomic sequencing to evaluate gut microbial changes.
- Assessment of cecal content, feces, liver tissue, and plasma samples.
Main Results:
- Bean-fed mice showed increased cecal bile acids and fecal excretion.
- Hepatic bile acid synthesis was elevated in bean-fed mice.
- Gut microbial composition shifted, increasing secondary bile acid metabolites (chenodeoxycholate/lithocholate) and decreasing others (hyocholate).
Conclusions:
- Cooked bean consumption confers resistance to diet-induced obesity and MASLD in mice.
- Beans modify bile acid metabolism by increasing hepatic synthesis and microbial diversity.
- Bean-induced alterations in bile acid profiles may improve dyslipidemia.