The Alleviating Effect of Abalone Viscera Collagen Peptide in DSS-Induced Colitis Mice: Effect on Inflammatory...

Abalone viscera is a discarded seafood by-product that contains a wealth of protein and is a good source of collagen peptides which have proven to have great potential in ameliorating host inflammation. The present study was conducted to evaluate the anti-inflammatory capacity of collagen peptide extracted from abalone viscera.

Low, medium, and high dosages (300, 600, and 900 mg/kg/d) of abalone viscera collagen peptide (AVCP) were orally administered to DSS-induced acute colitis mice. The inflammatory mediators and oxidative stress factors were assessed using the ELISA method, and gut microbiota was widely studied by 16S rRNA sequencing technology.

The results showed that oral administration of AVCP led to a significant alleviation of weight loss, colon length shortening, and DAI escalation in colitis mice. AVCP could also alleviate the pathological damage of colon tissue; inhibit splenic edema and thymic atrophy; reduce the serum level of inflammatory mediators (IL-1β, IL-6, TNF-α, IL-17A, and myeloperoxidase (MPO)); and improved antioxidant capacity (the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) increased and malondialdehyde (MDA) level decreased). Moreover, AVCP restored the balance of the gut microbiota, such as Escherichia-Shigella, Bacteroides, norank_f_Muribaculaceae, Rikenellaceae_RC9_gut_group, and Parasutterella.

Collectively, our observations elucidated the potential use of AVCP as a prebiotic for ulcerative colitis alleviation.