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Related Concept Videos

Blood Studies for Cardiovascular System I: Cardiac Biomarkers01:20

Blood Studies for Cardiovascular System I: Cardiac Biomarkers

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Cardiac biomarkers are enzymes, proteins, and hormones released into the blood when cardiac cells are injured. They are powerful tools for triaging.
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Troponins
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Cardiac biomarkers are critical in diagnosing, prognosing, and managing cardiovascular diseases. Routine measurement of specific biomarkers such as B-type natriuretic peptide (BNP), C-reactive protein (CRP), and homocysteine (Hcy) is common practice in clinical settings to evaluate heart function and predict cardiovascular events.
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
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  11. Cracking The Kinase Code: Urinary Biomarkers As Early Alarms For Aaa Rupture-a Pilot Study

Cracking the Kinase Code: Urinary Biomarkers as Early Alarms for AAA Rupture-A Pilot Study

Emma Maria Östling1, Tomas Baltrunas2,3, Nathalie Grootenboer1

  • 1Department of Vascular Surgery, Hospitalsenhed Midt, 8800 Viborg, Denmark.

Journal of Clinical Medicine
|June 13, 2025

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View abstract on PubMed

Summary
This summary is machine-generated.

Researchers identified six urinary tyrosine kinases as potential biomarkers for detecting ruptured abdominal aortic aneurysms (RAAA). This non-invasive approach could aid in early diagnosis and risk stratification for this life-threatening vascular condition.

Area of Science:

  • Biochemistry
  • Proteomics
  • Vascular Biology

Background:

  • Ruptured abdominal aortic aneurysm (RAAA) has a high mortality rate (nearly 90%).
  • Clinical need exists for biomarkers to identify high-risk individuals.
  • Non-invasive detection methods for abdominal aortic aneurysms (AAA) at risk of rupture are crucial.

Purpose of the Study:

  • To identify potential alterations in the urine proteome for non-invasive detection of AAA.
  • To discover urinary biomarkers for identifying AAA with a high risk of rupture.

Main Methods:

  • Utilized multiplexed kinase inhibitor beads (MIBs) and quantitative mass spectrometry (MIB/MS) for urine proteome analysis.
  • Employed iTRAQ labeling and LC-TEMPO MALDI-TOF/TOF for quantitative proteomic profiling.
  • Validated findings using Western blot and analyzed data with R/Bioconductor and open-source bioinformatics tools.
Keywords:
abdominal aortic aneurysmbiomarkerproteomerisk stratification

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Main Results:

  • Quantitatively measured activity of over 130 kinases, identifying significant dysregulation in EPHB6, AXL, EPHB4, DDR1, EPHA2, and EPHB3.
  • All identified significant proteins were tyrosine kinases, with a dominance of the Ephrin receptor type.
  • Observed reduced expression of specific kinases was confirmed via Western blot.

Conclusions:

  • Identified six dysregulated urinary tyrosine kinases as potential biomarkers for RAAA.
  • These findings suggest a promising, non-invasive approach for early AAA detection and risk assessment.
  • Further validation in larger prospective cohorts is necessary to confirm diagnostic utility and generalizability.
rupture
tyrosine kinase
urine