Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Liquid Biopsies
  11. The Development Of 3d Primary Co-culture Models Of The Human Airway

The Development of 3D Primary Co-Culture Models of the Human Airway

Cinta Iriondo1,2, Sem Koornneef1,2, Kari-Pekka Skarp1,2

  • 1Department of Pediatric Surgery, Sophia Children's Hospital, Erasmus Medical Center, 3015 CN Rotterdam, The Netherlands.

International Journal of Molecular Sciences
|June 13, 2025

Related Experiment Videos

Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids
10:12

Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids

Published on: March 23, 2022

8.1K
Author Spotlight: Modeling Human Airway Remodeling and Viral Responses Using Isogenic Epithelial, Endothelial, and Immune Cells
06:27

Author Spotlight: Modeling Human Airway Remodeling and Viral Responses Using Isogenic Epithelial, Endothelial, and Immune Cells

Published on: December 6, 2024

939
Generating 3D Spheres and 2D Air-Liquid Interface Cultures of Human Induced Pluripotent Stem Cell-Derived Type 2 Alveolar Epithelial Cells
07:02

Generating 3D Spheres and 2D Air-Liquid Interface Cultures of Human Induced Pluripotent Stem Cell-Derived Type 2 Alveolar Epithelial Cells

Published on: April 15, 2022

4.7K

View abstract on PubMed

Summary
This summary is machine-generated.

Developing a 3D lung model using human cells improves accuracy for clinical trials and personalized medicine. This advanced in vitro model better represents the human airway

Area of Science:

  • Pulmonary research
  • Biomedical engineering
  • Cell biology

Background:

  • Current animal and in vitro lung models fail to fully replicate human lung physiology and pathophysiology.
  • This limitation significantly hinders the translation of research findings into clinical practice and leads to clinical trial failures.
  • There is a critical need for improved in vitro models to enhance the prediction of clinical trial outcomes and advance personalized medicine.

Purpose of the Study:

  • To develop a more physiologically relevant 3D in vitro lung model using human primary cells.
  • To enhance the predictive capabilities of lung models for clinical applications and personalized medicine.

Main Methods:

  • Utilized the established air-liquid interface (ALI) culture system as a foundation.
  • Increased system complexity through co-culturing diverse primary lung cell types.
Keywords:
complex in vitro cultureshumanin vitro modellung

Related Experiment Videos

Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids
10:12

Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids

Published on: March 23, 2022

8.1K
Author Spotlight: Modeling Human Airway Remodeling and Viral Responses Using Isogenic Epithelial, Endothelial, and Immune Cells
06:27

Author Spotlight: Modeling Human Airway Remodeling and Viral Responses Using Isogenic Epithelial, Endothelial, and Immune Cells

Published on: December 6, 2024

939
Generating 3D Spheres and 2D Air-Liquid Interface Cultures of Human Induced Pluripotent Stem Cell-Derived Type 2 Alveolar Epithelial Cells
07:02

Generating 3D Spheres and 2D Air-Liquid Interface Cultures of Human Induced Pluripotent Stem Cell-Derived Type 2 Alveolar Epithelial Cells

Published on: April 15, 2022

4.7K
  • Tested various extracellular matrix coatings and incorporated a three-dimensional matrix.
  • Incorporated an endothelial layer to simulate vascularization.

    • Main Results:

    • Established a reproducible 3D in vitro model of the human airway.
    • The model features a differentiated mucociliary airway epithelium.
    • Included an underlying submucosa populated with fibroblasts.
    • Integrated an endothelial interface, mimicking key lung tissue components.

    Conclusions:

    • The developed 3D human lung model offers a more accurate representation of airway physiology compared to existing models.
    • This advanced model holds significant potential for improving preclinical drug testing and facilitating personalized medicine approaches.
    • Further refinement of this model can bridge the gap between in vitro research and clinical outcomes.
    primary cells