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  11. Morphological Alterations Of Conal Ridges And Differential Expression Of Ap2α In The Offspring Hearts Of Experimental Diabetic Rats

Morphological Alterations of Conal Ridges and Differential Expression of AP2α in the Offspring Hearts of Experimental Diabetic Rats

Tania Cristina Ramírez-Fuentes1,2, Ricardo Jaime-Cruz1,3,4, Carlos César Patiño-Morales1

  • 1Research Laboratory of Developmental Biology and Experimental Teratogenesis, Children's Hospital of México Federico Gomez, Mexico City 06720, Mexico.

International Journal of Molecular Sciences
|June 13, 2025

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View abstract on PubMed

Summary
This summary is machine-generated.

Maternal hyperglycemia during pregnancy can lead to congenital heart defects in offspring. This study shows that a high-glucose environment alters cardiac neural crest cell development, causing conotruncal heart abnormalities.

Area of Science:

  • Developmental Biology
  • Cardiovascular Science
  • Teratology

Background:

  • Neural crest cells (NCCs) are crucial for heart development, particularly the outflow tract.
  • Cardiac neural crest cells (cNCCs) are implicated in embryonic conus formation.
  • Maternal hyperglycemia may pose a teratogenic risk to fetal heart development.

Purpose of the Study:

  • To investigate the impact of a hyperglycemic intrauterine environment on conal ridge development and AP2α expression in offspring.
  • To identify specific cardiac malformations resulting from prenatal glucose exposure.

Main Methods:

  • Evaluation of morphological and anatomical changes in embryonic conal ridges.
  • Analysis of spatiotemporal expression of AP2α in offspring hearts at 13, 15, and 17 days post-coitum (DPC).
Keywords:
cardiac outflow tractsconal ridgesconotruncal cardiopathiesheart development

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  • Histological and immunostaining techniques were employed.

    • Main Results:

    • Offspring from the hyperglycemic group exhibited smaller heart dimensions compared to controls.
    • Altered AP2α immunostaining was observed in offspring hearts across all studied ages.
    • Conotruncal heart defects, including double outlet of the right ventricle and atrioventricular canal defects, were prevalent.

    Conclusions:

    • A hyperglycemic intrauterine environment significantly disrupts conal ridge development.
    • Prenatal glucose exposure is associated with increased incidence of conotruncal heart defects.
    • Understanding these mechanisms is vital for preventing congenital heart disease.
    hyperglycemia