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Related Concept Videos

Transmission-based Precautions II: Airborne and Protective Environment01:25

Transmission-based Precautions II: Airborne and Protective Environment

Transmission-based precautions are for patients infected or suspected to be infected (or colonized) with organisms posing a significant risk to others. The transmission precautions include airborne and protective environment precautions.
Airborne precautions:
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Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
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Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
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Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells
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Inhibiting Infectious Bronchitis Virus PLpro Using Ubiquitin Variants.

Vera J E van Vliet1,2, Olivia Roscow3, Kihun Kim4

  • 1Molecular Virology Laboratory, Leiden University Center for Infectious Diseases (LUCID), Leiden University Medical Center, 2333 ZA Leiden, The Netherlands.

International Journal of Molecular Sciences
|June 13, 2025
PubMed
Summary
This summary is machine-generated.

Novel ubiquitin variants (UbVs) show promise in combating infectious bronchitis virus (IBV) by inhibiting viral protease activity and enhancing innate immunity in chickens, offering a new antiviral strategy.

Keywords:
antiviralsavian infectious bronchitis viruscoronavirusesinnate immunityubiquitin variants

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Area of Science:

  • Virology
  • Immunology
  • Biotechnology

Background:

  • Infectious bronchitis virus (IBV) causes significant economic losses in the poultry industry due to its impact on egg production and increased susceptibility to secondary infections.
  • Current vaccines offer limited cross-protection against emerging IBV variants, highlighting the need for alternative control strategies like antiviral therapies.

Purpose of the Study:

  • To develop novel antiviral strategies against IBV by targeting its papain-like protease (PLpro).
  • To investigate the potential of ubiquitin variants (UbVs) as inhibitors of IBV PLpro's deubiquitinating activity and their impact on viral evasion of host immunity.

Main Methods:

  • Selection of high-affinity ubiquitin variants (UbVs) targeting IBV PLpro using phage display.
  • Assessment of UbVs' inhibitory effect on PLpro deubiquitinating activity.
  • Evaluation of viral inhibition in UbV-expressing cells using immunofluorescence microscopy.

Main Results:

  • Selected UbVs demonstrated high affinity for IBV PLpro.
  • UbVs successfully inhibited the deubiquitinating activity of PLpro, thereby hindering viral immune evasion.
  • Immunofluorescence microscopy revealed a significant reduction in IBV infection in UbV-expressing cells (approx. 5%) compared to control cells (approx. 30-40%).

Conclusions:

  • Ubiquitin variants represent a potent and innovative antiviral strategy against IBV.
  • Targeting IBV PLpro with UbVs shows potential for controlling IBV infections and mitigating economic losses in the poultry sector.