Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

  1. Home
  3. Transcriptome And Co-expression Network Analyses Of Resistant And Susceptible Rice Cultivars In Response To Meloidogyne Graminicola.
  1. Home
  3. Transcriptome And Co-expression Network Analyses Of Resistant And Susceptible Rice Cultivars In Response To Meloidogyne Graminicola.

Related Experiment Video

The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea
07:14

The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea

Published on: August 4, 2018

12.6K

Transcriptome and Co-Expression Network Analyses of Resistant and Susceptible Rice Cultivars in Response to

Shirui Zhang1,2, Zitong Xiao2,3, Kexiang Shen2,3

  • 1College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.

International Journal of Molecular Sciences
|June 13, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

Researchers screened rice cultivars for resistance to the damaging rice root-knot nematode (Meloidogyne graminicola). They identified key molecular pathways and genes involved in the plant

Keywords:
Meloidogyne graminicolaWGCNAhub genetranscriptome

More Related Videos

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
07:18

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

7.4K
Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize
06:12

Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize

Published on: September 15, 2023

1.4K

Related Experiment Videos

The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea
07:14

The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea

Published on: August 4, 2018

12.6K
Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
07:18

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

7.4K
Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize
06:12

Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize

Published on: September 15, 2023

1.4K

Area of Science:

  • Plant Pathology
  • Molecular Biology
  • Genetics

Background:

  • Meloidogyne graminicola is a major rice pathogen causing significant yield losses.
  • Varied resistance levels exist in rice germplasms, but underlying mechanisms are unclear.
  • Understanding resistance is crucial for developing resistant rice varieties.

Purpose of the Study:

  • To screen indica rice cultivars for resistance to Meloidogyne graminicola.
  • To elucidate the molecular mechanisms of differential host responses to M. graminicola infection.
  • To identify candidate genes and pathways associated with rice resistance.

Main Methods:

  • Screening of 122 indica rice cultivars for gall formation.
  • RNA sequencing of resistant (685) and susceptible (1008, 9311) cultivars at 5 days post-inoculation.
  • Differential gene expression analysis and Weighted Gene Co-expression Network Analysis (WGCNA).
  • Validation of candidate genes using RT-qPCR.

    • Main Results:

    • Indian indica accession 685 showed exceptional resistance, suppressing nematode development.
    • WGCNA and differential expression analysis identified key pathways: sugar metabolism, autophagic degradation, and phytohormone signal transduction.
    • Several candidate hub genes potentially involved in resistance were identified and validated.

    Conclusions:

    • This study provides insights into the molecular basis of rice resistance to Meloidogyne graminicola.
    • Identified pathways and genes offer targets for breeding resistant rice cultivars.
    • Highlights the importance of sugar metabolism and autophagy in plant-nematode interactions.