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Updated: Jun 16, 2025

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid

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Published on: February 27, 2019

Tripeptide-Loaded Liposomes as Multifunctional Components in Topical Formulations.

Michał Dymek¹, Maria José García-Celma², Elvira Escribano-Ferrer³

¹CUT Doctoral School, Faculty of Chemical Engineering and Technology, Cracow University of Technology, 31-155 Kraków, Poland.

International Journal of Molecular Sciences

|June 13, 2025

Summary

Researchers developed liposomes to deliver bioactive tripeptides for skin care. These liposomes enhanced peptide penetration into the epidermis, showing potential for anti-aging cosmetics.

Keywords:

bioactive tripeptides encapsulation liposomes skin permeation tyrosinase inhibitors

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Area of Science:

Dermocosmetics
Peptide Synthesis
Nanotechnology

Background:

Dermocosmetics utilize active substances and penetration enhancers for skin issues like hyperpigmentation.
Bioactive tripeptides with tyrosinase inhibition activity are key ingredients.
Liposomal delivery systems offer enhanced efficacy and targeted delivery.

Purpose of the Study:

To synthesize and characterize three bioactive tripeptides (CSF, CVL, CSN).
To encapsulate these tripeptides into liposomes and evaluate their physicochemical properties.
To assess the skin penetration and retention of liposome-encapsulated tripeptides.

Main Methods:

Solid-phase synthesis for tripeptide preparation.
In silico and in vitro elastase inhibition assays.
Liposome preparation and characterization (size, zeta potential, encapsulation efficiency).
Cytotoxicity assays on reconstructed human epidermis.
Ex vivo human skin penetration studies.

Main Results:

Synthesized tripeptides CSF, CVL, and CSN confirmed.
Liposomes (approx. 100 nm) were stable, non-cytotoxic, and achieved 20-30% encapsulation efficiency.
CSF tripeptide liposomes facilitated epidermal penetration and retention (4.65 ± 1.81 μg/cm²).
No significant transport into the receptor solution was observed.

Conclusions:

Liposomes are effective carriers for bioactive tripeptides.
The developed liposomal system enhances peptide penetration and retention within the epidermis.
These findings support the use of liposome-encapsulated tripeptides in anti-aging dermocosmetics.