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  11. Dual Regulatory Roles Of Slgamyb1 In Tomato Development: Ga-dependent And Ga-independent Mechanisms

Dual Regulatory Roles of SlGAMYB1 in Tomato Development: GA-Dependent and GA-Independent Mechanisms

Fanjia Zhong1, Fengpan Wang2, Zike Chen1

  • 1Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.

Plants (Basel, Switzerland)
|June 13, 2025

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View abstract on PubMed

Summary
This summary is machine-generated.

The study reveals that SlGAMYB1 in tomato impacts plant architecture by affecting gibberellin (GA) levels and cell proliferation. Overexpression of SlGAMYB1 influences internode elongation, leaf morphology, and floral organ number.

Area of Science:

  • Plant Biology
  • Molecular Genetics
  • Developmental Biology

Background:

  • The R2R3-MYB transcription factor GAMYB is vital for plant growth, but SlGAMYB1's specific roles in tomato are unclear.
  • Understanding SlGAMYB1 function is key to elucidating tomato development and yield regulation.

Purpose of the Study:

  • To investigate the biological functions of SlGAMYB1 in tomato development.
  • To analyze the effects of altered SlGAMYB1 activity on plant architecture, hormone levels, and gene expression.

Main Methods:

  • Overexpression of a miR159-resistant SlGAMYB1 construct (35S:SlGAMYB1m) in tomato.
  • Phenotypic analysis of transgenic plants, including growth, morphology, and floral organ number.
  • Gene expression analysis of key genes involved in gibberellin (GA) metabolism and cell cycle regulation.
Keywords:
SlGAMYB1floral organ formationgibberellinleaf development

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Main Results:

  • Overexpression of 35S:SlGAMYB1m resulted in dwarfism with reduced internode elongation due to decreased bioactive gibberellin (GA) levels.
  • SlGAMYB1 repressed SlGA3ox1 and activated SlGA2ox genes, altering GA homeostasis.
  • Leaf morphology was affected by inhibited cell proliferation via downregulation of cell cycle genes, and floral organ number increased, potentially through SlWUS upregulation.

Conclusions:

  • SlGAMYB1 regulates diverse tomato developmental processes through both GA-dependent and independent pathways.
  • Findings offer insights into GAMYB gene functional diversification and potential strategies for improving tomato architecture and yield.
tomato