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Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

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Related Experiment Video

Updated: Jun 16, 2025

CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System

CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System

Published on: September 23, 2021

Effective Agrobacterium-Mediated Transformation System for Eureka Lemon Using Whole Cotyledonary Node.

Jinfa Zhao¹, Yuan Chen¹, Jiajun Wang¹

¹Integrative Science Center of Germplasm Creation in Western China (Chongqing), Science City/Citrus Research Institute, Southwest University, Chongqing 400712, China.

Plants (Basel, Switzerland)

|June 13, 2025

Summary

Researchers optimized Agrobacterium-mediated transformation for lemons using whole cotyledonary nodes. This novel method significantly reduced tissue browning, enhancing transformation efficiency for creating transgenic lemon plantlets.

Keywords:

explant lemon transformation transformation efficiency whole cotyledonary node

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Published on: November 17, 2023

Area of Science:

Plant Biotechnology
Genetics
Horticulture

Background:

Agrobacterium-mediated transformation is crucial for citrus genetic modification.
Epicotyl explants in lemons suffer from severe browning, limiting transformation efficiency.
A need exists for improved explant sources for lemon genetic engineering.

Purpose of the Study:

To develop an optimized Agrobacterium-mediated transformation system for 'Eureka' lemon.
To address the challenge of tissue browning in lemon explants.
To enhance the efficiency of generating transgenic lemon plantlets.

Main Methods:

Utilized whole cotyledonary node explants from 'Eureka' lemon (Citrus limon).
Optimized Agrobacterium-mediated transformation protocols.
Evaluated tissue browning and transformation efficiency.

Main Results:

Significantly reduced tissue browning in explants.
Achieved a transformation efficiency of up to 14.48%.
Demonstrated a simple and rapid preparation method for cotyledonary nodes.

Conclusions:

The developed system using whole cotyledonary nodes is highly efficient for lemon transformation.
This method provides a novel and practical explant source for citrus genetic engineering.
Facilitated efficient generation of transgenic lemon plantlets.