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  5. Agricultural, Veterinary And Food Sciences
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  9. Horticultural Crop Improvement (incl. Selection And Breeding)
  11. The Impact Of Oserf34 On Rice Grain-processing Traits And Appearance Quality

The Impact of OsERF34 on Rice Grain-Processing Traits and Appearance Quality

Zhimin Du1,2, Yinan Jia1, Peisong Hu2

  • 1Rice Research Institute, College of Agronomy, Shenyang Agricultural University, Shenyang 110866, China.

Plants (Basel, Switzerland)
|June 13, 2025

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View abstract on PubMed

Summary
This summary is machine-generated.

ETHYLENE RESPONSIVE FACTOR34 (OsERF34) regulates rice grain quality. Overexpressing OsERF34 improves milling efficiency and head rice rate, offering a path for precision breeding.

Area of Science:

  • Plant Molecular Biology
  • Crop Quality Improvement
  • Agricultural Science

Background:

  • Head rice rate is crucial for rice quality but is often limited.
  • ETHYLENE RESPONSIVE FACTOR34 (OsERF34), an APETALA2 (AP2/ERF) transcription factor, is implicated in plant development.
  • Understanding OsERF34's role is key to enhancing rice processing quality.

Purpose of the Study:

  • To characterize the function of OsERF34 in rice grain morphology and processing quality.
  • To investigate the effects of OsERF34 knockout and overexpression on rice physicochemical properties.

Main Methods:

  • CRISPR/Cas9-mediated knockout (Oserf34) and overexpression (OsERF34-OE) in japonica rice (ZH11).
  • Analysis of grain morphology, chalkiness, starch properties, and milling resistance.
Keywords:
AP2/ERF transcription factorappearance qualityhead rice ratephysiochemical properties

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  • Evaluation of head rice rate and processing breakage.

    • Main Results:

    • Oserf34 mutants showed increased chalkiness, disordered starch granules, reduced amylose, and diminished milling resistance, leading to higher breakage and lower head rice rates.
    • Both mutants and OE lines exhibited grain narrowing, but OE lines displayed enhanced mechanical robustness.
    • OsERF34-OE lines achieved significantly higher head rice rates (6.5-7.1%) and improved milling quality.

    Conclusions:

    • OsERF34 acts as a dual-function regulator, influencing grain appearance and processing quality.
    • Overexpression of OsERF34 enhances grain mechanical properties and improves processing efficiency.
    • OsERF34 is a promising target for precision breeding to improve rice quality and processing.
    processing quality