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  11. Rejuvenation Of Mature Ilex Paraguariensis Plants Through Serial Rooted Cuttings: Exploring The Roles Of Mirnas In Reversing Adult Phase, Promoting Root Formation, And Determining Root Structure

Rejuvenation of Mature Ilex paraguariensis Plants Through Serial Rooted Cuttings: Exploring the Roles of miRNAs in Reversing Adult Phase, Promoting Root Formation, and Determining Root Structure

María J Duarte1, Raúl M Acevedo1, Nicolás L Ortiz1

  • 1Laboratorio de Biotecnología Aplicada y Genómica Funcional, Instituto de Botánica del Nordeste (IBONE-CONICET), Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, Sgto. Cabral 2131, Corrientes W3402BKG, Argentina.

Plants (Basel, Switzerland)
|June 13, 2025

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View abstract on PubMed

Summary
This summary is machine-generated.

MicroRNAs regulate plant development. Specific microRNAs (miRNAs) like miR156/157/528 and miR172 control the transition from juvenile to adult phases in Ilex paraguariensis, aiding propagation.

Area of Science:

  • Plant Molecular Biology
  • Developmental Biology
  • Agricultural Science

Background:

  • Plant phase change from juvenile to adult involves microRNAs (miRNAs).
  • This transition is crucial for woody plant propagation, especially for recalcitrant species like Ilex paraguariensis.
  • Understanding these molecular mechanisms can improve mass propagation efficiency.

Purpose of the Study:

  • To identify conserved miRNAs differentially expressed between juvenile and adult Ilex paraguariensis.
  • To investigate the role of these miRNAs in vegetative phase change and rejuvenation.
  • To explore the potential of miRNA analysis for enhancing Ilex paraguariensis mass propagation.

Main Methods:

  • High-throughput sequencing of small RNA libraries from rejuvenated and mature Ilex paraguariensis plants.
Keywords:
Ilex paraguariensisadventitious rootingmiRNAsrejuvenation

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  • Differential expression analysis of conserved miRNAs.
  • Correlation of miRNA expression patterns with plant developmental stages and rejuvenation characteristics.

    • Main Results:

    • miR156/miR157/miR528 expression was high in juvenile plants and decreased with maturation.
    • miR172 expression was predominant in adult plants.
    • Rejuvenation via serial-rooted cuttings led to the re-expression of juvenile phase characteristics, including improved adventitious root formation and plant vigour.

    Conclusions:

    • Conserved miRNAs, particularly miR156/157/528 and miR172, play a key role in regulating vegetative phase change in Ilex paraguariensis.
    • Rejuvenation in Ilex paraguariensis is associated with a shift in miRNA expression towards a juvenile state.
    • Analyzing miRNA profiles can facilitate early identification of rejuvenated material, enhancing mass propagation of Ilex paraguariensis and other recalcitrant woody species.