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  11. Genome-wide Dissection Of Sorghum B3 Transcription Factor Family Identifies Sblav1 As A Critical Transcriptional Regulator Of Starch Biosynthesis In Developing Sorghum Grains

Genome-Wide Dissection of Sorghum B3 Transcription Factor Family Identifies SbLAV1 as a Critical Transcriptional Regulator of Starch Biosynthesis in Developing Sorghum Grains

Xiangling Gong1, Jing Li1, Zheyu Yan1

  • 1College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China.

Plants (Basel, Switzerland)
|June 13, 2025

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View abstract on PubMed

Summary
This summary is machine-generated.

Sorghum B3 transcription factors (TFs) are key to grain development and starch biosynthesis. The SbLAV1 gene, part of this family, activates genes essential for starch production in sorghum.

Area of Science:

  • Plant Molecular Biology
  • Genetics and Genomics

Background:

  • Sorghum (Sorghum bicolor L.) is a globally significant cereal crop with diverse applications.
  • Limited understanding exists regarding the genetic regulation of sorghum grain development and starch biosynthesis.
  • B3 family transcription factors (TFs) are known regulators of plant development, including grain processes.

Purpose of the Study:

  • To conduct a comprehensive analysis of the B3 gene family in sorghum.
  • To investigate the role of B3 TFs in sorghum grain development and starch biosynthesis.

Main Methods:

  • Genome-wide identification and classification of sorghum B3 genes.
  • Analysis of cis-regulatory elements in the upstream regions of B3 genes.
  • Gene expression profiling across various sorghum tissues.
Keywords:
B3 transcription familySbLAV1Sorghum (Sorghum bicolor L.)starch biosynthesis

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  • Functional validation of SbLAV1's role in starch biosynthesis gene regulation.

    • Main Results:

    • Identified 76 sorghum B3 genes distributed across 10 chromosomes, classified into four subfamilies (ARF, RAV, LAV, REM).
    • Discovered diverse cis-regulatory elements (light, hormone, growth, stress) in B3 gene promoters.
    • Observed distinct expression patterns for B3 genes in different tissues.
    • SbLAV1 (LAV subfamily) co-expressed with 15 starch biosynthesis-related genes (SBRGs) and activated their promoters.

    Conclusions:

    • Sorghum B3 family members, particularly SbLAV1, are likely crucial regulators of starch biosynthesis and grain development.
    • This study provides foundational insights into the molecular mechanisms governing sorghum grain quality.
    transcriptional regulation