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Related Concept Videos

Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...

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Complete Genome Sequencing of a G3P[14] Rabbit Rotavirus.

Ahmed Hassan Omar1, Francesco Pellegrini1, Cristiana Catella1

  • 1Department of Veterinary Medicine, University of Bari Aldo Moro, 70010 Valenzano, Italy.

Animals : an Open Access Journal From MDPI
|June 13, 2025
PubMed
Summary
This summary is machine-generated.

A new Group A rotavirus (RVA) strain was identified in rabbits in Italy. This lapine RVA strain shares genetic similarities with other rabbit and human rotaviruses, highlighting potential zoonotic links.

Keywords:
G3P[14]enteritisgenome reconstructionrabbitrotavirus

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Area of Science:

  • Veterinary Virology
  • Molecular Epidemiology
  • Zoonotic Diseases

Background:

  • Group A rotaviruses (RVAs) are significant causes of acute dehydrating diarrhea in young animals and humans globally.
  • In rabbits, RVAs contribute to enteric disease, often alongside other pathogens.
  • Previous studies suggest potential zoonotic transmission of RVAs between species.

Purpose of the Study:

  • To identify and characterize a novel lapine (rabbit) RVA strain from an Italian rabbit breeding farm.
  • To determine the complete genome and genotype constellation of the identified RVA strain.
  • To investigate the phylogenetic relationship of the lapine RVA with known RVA strains from rabbits and humans.

Main Methods:

  • Quantitative reverse transcription-polymerase chain reaction (RT-PCR) for RVA detection in intestinal contents.
  • Polymerase chain reaction (PCR)-based enrichment protocol for viral genome amplification.
  • Nanopore sequencing for whole-genome reconstruction of the RVA strain.
  • Phylogenetic analysis to determine genetic relatedness with other RVA strains.

Main Results:

  • A lapine RVA strain, Rabbit-wt/ITA/36-9/2020/G3P[14], was identified in rabbits with increased mortality and enteric symptoms.
  • The nearly complete 11-gene segment genome was reconstructed, showing a genotype constellation (G3-P[14]-I2-R2-C2-M3-A9-N2-T6-E5-H3) consistent with known lapine RVAs.
  • Phylogenetic analysis revealed high nucleotide identity with other lapine RVA strains and two Belgian human G3P[14] strains of presumed zoonotic origin.
  • The NSP2 gene exhibited closer clustering with rare human G3P[9] strains, suggesting complex evolutionary pathways.

Conclusions:

  • The study reports a novel G3P[14] lapine RVA strain from Italy with genetic links to other animal and human RVAs.
  • The findings support the zoonotic potential of lapine RVAs and underscore the importance of monitoring animal rotaviruses.
  • Understanding the evolutionary history of RVAs in different mammalian hosts is crucial for public health and disease control strategies.