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Updated: Jun 16, 2025

Intranasal Immunization and Milk Collection in Studies of Maternal Immunization in New Zealand White Rabbits Oryctolagus cuniculus
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The Relationship Between Protein Fraction Contents and Immune Cells in Milk.

Haitong Wang1,2, Xiaoli Ren1,2,3, Li Liu1,2,4

  • 1Frontiers Science Center for Animal Breeding and Sustainable Production, Huazhong Agricultural University, Wuhan 430070, China.

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Summary

Somatic cell counts indicate mammary gland health, with mastitis altering milk protein fractions. This study reveals how lactation stage, parity, and mastitis severity affect key milk proteins, offering insights for dairy farming.

Keywords:
cowdifferential somatic cell count (DSCC)immune cell countmammary gland healthmilk protein fractionsomatic cell score (SCS)

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Area of Science:

  • Dairy Science
  • Animal Health
  • Spectroscopy

Background:

  • Mastitis significantly impacts milk yield and quality.
  • Somatic cell count (SCC) and differential somatic cell count (DSCC) are key indicators of mammary gland health.

Purpose of the Study:

  • To analyze milk protein fraction secretion patterns.
  • To investigate variations under mastitis conditions.
  • To associate protein fractions with immune cell counts.

Main Methods:

  • Utilized mid-infrared spectroscopy to predict 8 milk protein fractions.
  • Applied linear mixed models to analyze secretion patterns and associations.
  • Studied 21,388 milk samples from 33 herds.

Main Results:

  • Lactation stage and parity influenced protein fractions (e.g., IgG, casein, whey proteins).
  • Mastitis altered milk yield, fat, protein percentages, and specific protein fractions (e.g., increased IgG, β-LG).
  • Somatic cell score (SCS) and DSCC showed complex correlations with milk composition and protein fractions.

Conclusions:

  • Mastitis significantly alters milk protein profiles, impacting quality.
  • Understanding these changes aids in early mastitis diagnosis and research.
  • Findings support sustainable dairy farming practices and improved milk production.