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  11. Rose Bengal Conjugated To Lectins For Targeted Antibacterial Photodynamic Treatment

Rose Bengal Conjugated to Lectins for Targeted Antibacterial Photodynamic Treatment

Melad Atrash1, Iryna Hovor1, Marina Nisnevitch1

  • 1Department of Chemical Engineering, Ariel University, Kiryat-ha-Mada, Ariel 40700, Israel.

Molecules (Basel, Switzerland)
|June 13, 2025

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View abstract on PubMed

Summary
This summary is machine-generated.

Researchers developed a new photodynamic antibacterial chemotherapy by linking Rose Bengal (RB) to lectins. Concanavalin A-RB conjugates show enhanced efficacy against both Gram-positive and Gram-negative bacteria, offering a promising alternative to antibiotics.

Area of Science:

  • Biochemistry
  • Microbiology
  • Photochemistry

Background:

  • Rising antibiotic resistance necessitates novel antibacterial strategies.
  • Photodynamic antibacterial chemotherapy (PACT) offers an alternative treatment modality.
  • Photosensitizer conjugation to targeting molecules can enhance PACT efficacy.

Purpose of the Study:

  • To conjugate the photosensitizer Rose Bengal (RB) to lectins Concanavalin A (ConA) and Wheat Germ Agglutinin (WGA).
  • To evaluate the efficacy of these lectin-RB conjugates against Gram-positive and Gram-negative bacteria using PACT.
  • To compare the antibacterial activity of ConA-RB and WGA-RB with free RB.

Main Methods:

  • Covalent conjugation of RB to ConA and WGA.
  • Spectroscopic and chromatographic analysis to confirm conjugation.
Keywords:
Rose Bengalconcanavalin Aconjugationlectins

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  • Microscopic examination for bacterial agglutination.
  • Photodynamic antibacterial assays against *S. aureus*, *E. coli*, *P. aeruginosa*, and *S. paratyphi* B.

    • Main Results:

    • Successful conjugation of RB to ConA and WGA was confirmed.
    • ConA demonstrated stronger agglutination of Gram-positive and Gram-negative bacteria compared to WGA.
    • ConA-RB achieved complete eradication of *S. aureus* at lower concentrations and light doses than free RB or WGA-RB.
    • ConA-RB showed improved efficacy against Gram-negative bacteria compared to free RB.
    • WGA-RB exhibited preferential activity against *S. aureus*.

    Conclusions:

    • Lectin-photosensitizer conjugates, particularly ConA-RB, enhance PACT efficacy against a range of bacteria.
    • This approach offers a promising strategy for targeted antibacterial therapy.
    • The study highlights the potential of lectin-RB conjugates as an alternative to conventional antibiotics.
    photodynamic antibacterial activity
    photosensitizers