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  1. Home
  3. Rapid Bactericidal Activity Of Punica Granatum L. Peel Extract: A Natural Alternative For Mastitis Prevention In Dairy Cattle.
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  3. Rapid Bactericidal Activity Of Punica Granatum L. Peel Extract: A Natural Alternative For Mastitis Prevention In Dairy Cattle.

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Rapid Bactericidal Activity of Punica granatum L. Peel Extract: A Natural Alternative for Mastitis Prevention in

Carenn Rodrigues E Almeida Silva1, Camila Silva Vidal1, Sergio Martins de Andrade Filho1

  • 1Department of Biology, Rio de Janeiro State University, Rio de Janeiro 23070-200, Brazil.

Molecules (Basel, Switzerland)
|June 13, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

Pomegranate peel extract shows potent antibacterial effects against mastitis pathogens like E. coli and S. aureus. This natural alternative offers rapid action, comparable to conventional disinfectants, for dairy farm hygiene.

Keywords:
bovine mastitisdisinfectantsplant-based antimicrobialpre- and post-dipping

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Area of Science:

  • Agricultural Science
  • Microbiology
  • Natural Product Chemistry

Background:

  • Rising bacterial resistance to conventional disinfectants and antibiotics necessitates novel solutions in dairy farming.
  • Bovine mastitis remains a significant challenge, impacting animal health and milk production.

Purpose of the Study:

  • To evaluate the bactericidal efficacy of pomegranate (Punica granatum L.) peel ethanolic extract.
  • To assess its potential as a natural alternative for mastitis prevention in dairy cattle, particularly in pre- and post-dipping applications.

Main Methods:

  • The study assessed the extract's activity against Escherichia coli and Staphylococcus aureus.
  • Membrane disruption was measured via propidium iodide uptake and extracellular DNA release.
  • Phytochemical analysis identified key bioactive compounds, with punicalagin being predominant.

Main Results:

  • Pomegranate peel extract demonstrated rapid membrane disruption within 30 seconds at 10 mg/mL.
  • Complete bacterial growth inhibition was observed at 25 and 50 mg/mL within 30 seconds.
  • Efficacy was comparable to commercial chlorhexidine and hypochlorite disinfectants.

Conclusions:

  • Punica granatum peel extract is a fast-acting bactericidal agent with significant potential for controlling mastitis pathogens.
  • Its natural origin and high efficacy make it a promising alternative to synthetic disinfectants in the dairy industry.