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  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Medical Biochemistry And Metabolomics
  9. Medical Biochemistry - Amino Acids And Metabolites
  11. Development And Validation Of A Simple And Cost-effective Lc-ms/ms Method For The Quantitation Of The Gut-derived Metabolite Trimethylamine N-oxide In Human Plasma Of Healthy And Hyperlipidemic Volunteers.
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Medical Biochemistry And Metabolomics
  9. Medical Biochemistry - Amino Acids And Metabolites
  11. Development And Validation Of A Simple And Cost-effective Lc-ms/ms Method For The Quantitation Of The Gut-derived Metabolite Trimethylamine N-oxide In Human Plasma Of Healthy And Hyperlipidemic Volunteers.

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Development and Validation of a Simple and Cost-Effective LC-MS/MS Method for the Quantitation of the Gut-Derived Metabolite Trimethylamine N-Oxide in Human Plasma of Healthy and Hyperlipidemic Volunteers.

Nikolaos A Parisis1, Panoraia Bousdouni2, Aikaterini Kandyliari2,3

  • 1Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece.

Molecules (Basel, Switzerland)
|June 13, 2025

View abstract on PubMed

Summary
This summary is machine-generated.
Keywords:
Rhodamine BTMAObiomarkershuman plasma

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A new analytical method accurately measures Trimethylamine N-oxide (TMAO) in human blood plasma. This reliable and sensitive technique aids in diagnosing diseases linked to elevated TMAO levels.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Clinical Diagnostics

Background:

  • Trimethylamine N-oxide (TMAO) is a metabolite linked to cardiovascular disease, diabetes, kidney disease, neurological disorders, and cancer.
  • Accurate quantification of TMAO in human plasma is crucial for disease diagnosis and monitoring.
  • Existing analytical methods require improvement in terms of reliability, cost-effectiveness, and sensitivity.

Purpose of the Study:

  • To develop and validate a sensitive, specific, and accurate LC-MS/MS method for determining TMAO in human blood plasma.
  • To establish a cost-effective and reliable analytical approach for TMAO quantification.
  • To apply the validated method to analyze plasma samples from healthy and hyperlipidemic individuals.

Main Methods:

  • Development and validation of a Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) method.
liquid chromatography
method validation
simple extraction methodology
tandem mass spectrometry
  • Simple sample preparation involving protein precipitation and a non-deuterated internal standard (IS).
  • Analysis using a triple quadrupole mass spectrometer and adaptation to UPLC-QTOF/MS, validated per EMA and FDA guidelines.

    • Main Results:

    • The developed LC-MS/MS method demonstrated high specificity, sensitivity, and accuracy for TMAO determination in human plasma.
    • A lower limit of quantification (LLOQ) of 0.25 µM was achieved.
    • The method exhibited acceptable intra- and inter-assay precision and trueness, and was successfully applied to volunteer plasma samples.

    Conclusions:

    • A validated LC-MS/MS method provides a reliable, sensitive, and accurate means for quantifying TMAO in human plasma.
    • This method supports clinical research and diagnostics for TMAO-associated diseases.
    • The developed assay is suitable for routine analysis in clinical and research settings.