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  1. Home
  3. Study On The Synchronous Removal Of Nitrogen And Phosphorus By Autotrophic/heterotrophic Denitrification In The Presence Of Pyrite.
  1. Home
  3. Study On The Synchronous Removal Of Nitrogen And Phosphorus By Autotrophic/heterotrophic Denitrification In The Presence Of Pyrite.

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Study on the Synchronous Removal of Nitrogen and Phosphorus by Autotrophic/Heterotrophic Denitrification in the

Minyi Zhu1, Minhui Ma1, Shuo Chen1

  • 1Department of Environmental Science and Engineering, School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China.

Molecules (Basel, Switzerland)
|June 13, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

Natural pyrite effectively removes nitrogen and phosphorus from water, mitigating eutrophication. This study details optimal conditions and microbial roles for enhanced water quality, offering a cost-effective solution.

Keywords:
autotrophic denitrificationbiofiltermicrobial characteristicsnitrogen and phosphorus removalpyrite

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Area of Science:

  • Environmental Science
  • Water Treatment
  • Microbiology

Background:

  • Nutrient pollution, particularly nitrogen (N) and phosphorus (P), drives water eutrophication.
  • Conventional biological treatments struggle to meet stringent effluent standards for N and P removal.
  • Autotrophic denitrification offers a promising, low-cost method for simultaneous N and P removal with minimal sludge.

Purpose of the Study:

  • To investigate the efficacy of natural pyrite in a denitrification system for simultaneous nitrogen and phosphorus removal.
  • To elucidate the mechanisms governing N and P removal using pyrite.
  • To determine the influence of operational parameters and microbial communities on treatment performance.

Main Methods:

  • Utilized natural pyrite in a denitrification system to treat N and P.
  • Varied empty bed contact time (EBCT) and pH to assess their impact on removal efficiency.
  • Analyzed the effect of carbon source addition and C/N ratio on synergistic denitrification.
  • Conducted microbial community analysis to identify key microbial players.

    • Main Results:

    • Optimal NO3--N removal of 90.24% achieved at 8 h EBCT.
    • Optimal PO43--P removal of 81.58% achieved at 12 h EBCT.
    • Carbon source addition and increased C/N ratio significantly enhanced phosphorus removal.
    • Identified key microbial phyla (Chlorobiota, Bacteroidota, Chloroflexota) and genera (Thauera, Aridibacter, Gemmatimonas, Thiobacillus, Rhodoplanes, Geobacter) involved.

    Conclusions:

    • Natural pyrite is an effective medium for simultaneous nitrogen and phosphorus removal in denitrification systems.
    • EBCT and pH are critical parameters influencing treatment efficiency.
    • Synergistic autotrophic/heterotrophic denitrification, enhanced by carbon sources, is crucial for optimal P removal.
    • Specific microbial communities are vital for the efficient removal of N and P.