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Updated: Jun 15, 2025
Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens
Published on: February 24, 2021
Identification of three novel mite allergens, Der f 42, Der f 43, and Der f 44, from Dermatophagoides farinae by
Ze-Lang Cai1, Shan Liu1, Anyi Ji2
1Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Shenzhen University Medical School, Shenzhen 518060, China.
Background:
House dust mites (HDMs) produce major inhaled allergens that trigger allergic diseases worldwide. The identities of the full spectrum of HDM allergenic components are not yet known. We aimed to develop a new gelsolin interactome-analysis (GIA) method for discovering and identifying novel allergens.
Methods:
Gelsolin-binding proteins (GBPs) in Dermatophagoides farinae extracts were analyzed with gelsolin-affinity resin and LC-MS/MS (liquid chromatography coupled to tandem mass spectrometry) analyses. Recombinant proteins generated from cDNAs encoding candidate allergens were expressed in a prokaryotic system. IgE binding was evaluated by ELISA (enzyme-linked immunosorbent assay), western blotting, and dot-blotting.
Results:
A total of 14 GBPs were assayed, including 10 known allergens and 4 candidates. Three candidates bound recombinant gelsolin, and they were named Der f 42, Der f 43, and Der f 44 by the WHO/IUIS Allergen Nomenclature Sub-committee. IgE-binding assays showed that Der f 42, Der f 43, and Der f 44 had IgE-binding rates of 7.2% (9/125), 8.5% (12/143), and 6.7% (6/90), respectively.
Conclusion:
GIA revealed 3 novel HDM proteins in this study and represents a new strategy for discovering and studying allergens.
